Anti-alpha 1 Spectrin antibody [17C7]

• 产品名称

  Anti-alpha 1 Spectrin抗体[17C7]
  参阅全部 alpha 1 Spectrin 一抗

• 描述

  小鼠单克隆抗体[17C7] to alpha 1 Spectrin

• 宿主

  Mouse

• 特异性

  ab11751 is developed against the alpha I spectrin SH3 domain. It does not crossreact with alpha II (nonerythroid) spectrin SH3 domain or recombinant human fodrin SH3 domain. In NIH 3T3 cells, ab11751 reacts with a 200 kDa protein which is a candidate marker of endocytic vesicles called macropinosomes.

• 经测试应用

  适用于: ELISA, IHC-Fr, ICC/IF, WBmore details

• 种属反应性

  与反应: Mouse, Rat, Human
  

• 免疫原

  Recombinant alpha I spectrin (erythroid spectrin) SH3 domain.

• 阳性对照

• Erythroid spectrin in red blood cells and cerebellum.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液

  Constituent: PBS

• 浓度

• 100 µl 浓度为 1 mg/ml

• 纯度

  Affinity purified

• 纯化说明

  Purified from tissue culture supernatant.

• 克隆

  单克隆

• 克隆编号

  17C7

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab11751于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 6708 Human

• Entrez Gene: 20739 Mouse

• Entrez Gene: 289257 Rat

• Omim: 182860 Human

• SwissProt: P02549 Human

• SwissProt: P08032 Mouse

• Unigene: 119825 Human

• Unigene: 200611 Mouse

• 别名

• Elliptocytosis 2 antibody

• Elliptocytosis2 antibody

• Erythrocyte alpha spectrin antibody

• erythrocyte antibody

• Erythroid alpha spectrin antibody

• Erythroid alpha-spectrin antibody

• Erythroid spectrin alpha antibody

• HPP antibody

• HS3 antibody

• Spectrin alpha chain antibody

• Spectrin alpha chain erythrocyte antibody

• Spectrin alpha erythrocytic 1 antibody

• SPH3 antibody

• SPTA 1 antibody

• SPTA antibody

• SPTA1 antibody

• SPTA1_HUMAN antibody

• Alpha I spectrin antibody

• EL 2 antibody

• EL2 antibody

• 

  Western blot - Anti-alpha 1 Spectrin antibody [17C7] (ab11751)

  All lanes : Anti-alpha 1 Spectrin antibody [17C7] (ab11751) at 1 µg/ml
  
  Lane 1 : MW marker
  Lane 2 : Human Lung lysate
  Lane 3 : Mouse Lung lysate
  Lane 4 : Rat Brain lysate
  Lane 5 : Human Kidney lysate
  
  Lysates/proteins at 50 µg per lane.
  
  Secondary
  All lanes : HRP labeled goat anti-mouse IgG
  
  Predicted band size: 280 kDa
  
  

• 

  Western blot - Anti-alpha 1 Spectrin antibody [17C7] (ab11751)This image is courtesy of an Abreview submitted by Dr. Mahesh Shivananjappa

  Anti-alpha 1 Spectrin antibody [17C7] (ab11751) at 1/1000 dilution + Human Platelet whole cell lysate at 25 µg
  
  Secondary
  HRP-conjugated Goat Anti-mouse IgG at 1/10000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 280 kDa
  Additional bands at: 200 kDa (possible cleavage fragment)
  
  
  Exposure time: 1 hour
  

  See Abreview