Anti-IRAK2抗体
参阅全部 IRAK2 一抗
兔多克隆抗体to IRAK2
Rabbit
Anti-IRAK2 has no cross response to IRAK.
适用于: ICC/IF, WBmore details
与反应: Human
Synthetic peptide, corresponding to amino acids 546-564 of Human IRAK2.
The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK2 (1). Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-kB activation. IRAKs also associate with Toll-like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kB activation (2,3).Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. IRAK2 is expressed in a variety of human tissues.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.2
Preservative: 0.02% Sodium azide
浓度
100 µg 浓度为 1 mg/ml
Affinity purified
Purified IgG was prepared by affinity chromatography.
The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK2 (1). Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-kB activation. IRAKs also associate with Toll-like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kB activation (2,3).Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. IRAK2 is expressed in a variety of human tissues.
多克隆
IgG
Abpromise™承诺保证使用ab6148于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use a concentration of 2 µg/ml. | |
| WB | 1/500 - 1/1000. Predicted molecular weight: 65 kDa. |
Entrez Gene: 3656 Human
Omim: 603304 Human
SwissProt: O43187 Human
Unigene: 449207 Human
Interleukin-1 receptor-associated kinase-like 2 antibody
IRAK 2 antibody
IRAK-2 antibody
Irak2 antibody
IRAK2_HUMAN antibody
MGC150550 antibody
il1rak2 antibody
Interleukin 1 receptor associated kinase 2 antibody
Interleukin 1 receptor associated kinase like 2 antibody
Immunocytochemistry/ Immunofluorescence - Anti-IRAK2 antibody (ab6148)
Immunofluorescent analysis of Hela cells staining IRAK2 with ab6148 at 20µg/ml
Western blot - Anti-IRAK2 antibody (ab6148)
All lanes : Anti-IRAK2 antibody (ab6148) at 2 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : K562 whole cell lysate
Predicted band size: 65 kDa
Observed band size: 65 kDa
Immunocytochemistry/ Immunofluorescence - Anti-IRAK2 antibody (ab6148)
ab6148 at 2µg/ml staining IRAK2 in Hela cells by ICC/IF
Immunocytochemistry/ Immunofluorescence - Anti-IRAK2 antibody (ab6148)
Immunofluorescence of IRAK-2 in Hela cells using ab6148 at 10 ug/ml.
