Anti-hnRNP A2B1 antibody [DP3B3]

• 产品名称

  Anti-hnRNP A2B1抗体[DP3B3]
  参阅全部 hnRNP A2B1 一抗

• 描述

  小鼠单克隆抗体[DP3B3] to hnRNP A2B1

• 宿主

  Mouse

• 经测试应用

  适用于: IHC-Pmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Recombinant full length protein (His-tag) corresponding to Human hnRNP A2B1. N-terminal His tag.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.1% Sodium azide
  Constituent: PBS

• 纯度

  Protein A purified

• 纯化说明

  Purified from supernatant.

• 克隆

  单克隆

• 克隆编号

  DP3B3

• 骨髓瘤

  Sp2/0

• 同种型

  IgG2a

The Abpromise guarantee

Abpromise™承诺保证使用ab6102于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 3181 Human

• Omim: 600124 Human

• SwissProt: P22626 Human

• Unigene: 487774 Human

• 别名

• Heterogeneous nuclear ribonucleoproteins A2/B1 antibody

• hnRNP A2 / hnRNP B1 antibody

• hnRNP A2 antibody

• hnRNP A2/B1 antibody

• hnRNP B1 antibody

• hnRNP-A2 antibody

• hnRNP-B1 antibody

• hnRNPA2 antibody

• Hnrnpa2b1 antibody

• hnRNPB1 antibody

• HNRPA2 antibody

• HNRPA2B1 antibody

• HNRPB1 antibody

• Nuclear ribonucleoprotein particle A2 protein antibody

• RNP A2 antibody

• RNP B1 antibody

• RNP-A2 antibody

• RNP-B1 antibody

• RNPA2 antibody

• RNPB1 antibody

• ROA2_HUMAN antibody

• SNRPB1 antibody

• Heterogeneous nuclear ribonucleoprotein A2 antibody

• Heterogeneous nuclear ribonucleoprotein A2/B1 antibody

• Heterogeneous nuclear ribonucleoprotein B1 antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A2B1 antibody [DP3B3] (ab6102)

  ab6102 (1µg/ml) staining hnRNP A2B1 in human liver.
  Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A2B1 antibody [DP3B3] (ab6102)

  ab6102 (1µg/ml) staining hnRNP A2B1 in human caecum, using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining in the mucosal epithelium.
  Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.