Anti-Endothelin 1 antibody [TR.ET.48.5]

• 产品名称

  Anti-Endothelin 1抗体[TR.ET.48.5]
  参阅全部 Endothelin 1 一抗

• 描述

  小鼠单克隆抗体[TR.ET.48.5] to Endothelin 1

• 宿主

  Mouse

• 特异性

  Immunohistochemical staining of ET-1 in human corpus cavernosum tissue with this antibody results in staining of endothelial cells. Radioimmune assays can be used to concentrate ET-1 in solution (e.g. serum/plasma, milk, urine).

• 经测试应用

  适用于: Flow Cyt, ICC/IF, IHC-P, WBmore details

• 种属反应性

  与反应: Mouse, Rat, Human
  

• 免疫原

  Full length native protein (purified) corresponding to Human Endothelin 1 conjugated to keyhole limpet haemocyanin.

• 表位

  Studies suggest that this antibody binds to an epitope in the region of ET-1 represented by amino acids 8-16.

• 阳性对照

• human bowel

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.05% Sodium azide
  Constituent: PBS

• 浓度

• 100 µl 浓度为 2.5 mg/ml

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  TR.ET.48.5

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab2786于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 1906 Human

• Entrez Gene: 13614 Mouse

• Entrez Gene: 24323 Rat

• Omim: 131240 Human

• SwissProt: P05305 Human

• SwissProt: P22387 Mouse

• SwissProt: P22388 Rat

• Unigene: 511899 Human

• Unigene: 713645 Human

别名

• ARCND3 antibody

• Big endothelin-1 antibody

• EDN1 antibody

• EDN1_HUMAN antibody

• ET-1 antibody

• ET1 antibody

• HDLCQ7 antibody

• PPET1 antibody

• Preproendothelin 1 antibody

• Preproendothelin-1 antibody

• QME antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Endothelin 1 antibody [TR.ET.48.5] (ab2786)

  ab2786 labelling Endothelin 1 (green) in the secretion of HeLa cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.

• 

  Western blot - Anti-Endothelin 1 antibody [TR.ET.48.5] (ab2786)

  Anti-Endothelin 1 antibody [TR.ET.48.5] (ab2786) at 1/500 dilution + PC12 cell lysate at 25 µg
  
  Predicted band size: 24 kDa
  Observed band size: 30 kDawhy is the actual band size different from the predicted?
  
  

• 

  Flow Cytometry - Anti-Endothelin 1 antibody [TR.ET.48.5] (ab2786)

  Flow cytometry analysis of Endothelin 1 showing positive staining in the cytoplasm of 293T cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde, washed with PBS, and incubated with ab2786 (0.5 ug/test) for 60 min at room temperature. Cells were then blocked in a solution of 2% BSA-PBS for 30 min at room temperature, incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody, and re-suspended in PBS for FACS analysis.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Endothelin 1 antibody [TR.ET.48.5] (ab2786)

  ab2786 labelling Endothelin 1 (green) in the secretion of PC12 cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.

• 

  Flow Cytometry - Anti-Endothelin 1 antibody [TR.ET.48.5] (ab2786)

  Flow cytometry analysis of Endothelin 1 showing positive staining in the cytoplasm of HepG2 cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde, washed with PBS, and incubated with ab2786 (0.5 ug/test) for 60 min at room temperature. Cells were then blocked in a solution of 2% BSA-PBS for 30 min at room temperature, incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody, and re-suspended in PBS for FACS analysis.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Endothelin 1 antibody [TR.ET.48.5] (ab2786)

  ab2786 labelling Endothelin 1 (green) in the secretion of HUVEC cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-mouse IgG (H+L)  was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.

• 

  Flow Cytometry - Anti-Endothelin 1 antibody [TR.ET.48.5] (ab2786)

  Flow cytometry analysis of Endothelin 1 showing positive staining in the cytoplasm of 3T3 cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde, washed with PBS, and incubated with ab2786 (0.5 ug/test) for 60 min at room temperature. Cells were then blocked in a solution of 2% BSA-PBS for 30 min at room temperature, incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody, and re-suspended in PBS for FACS analysis.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin 1 antibody [TR.ET.48.5] (ab2786)

  Figure 1 and Figure 2 show immunolocalization of ET-1 in human bowel using ab2786.