Anti-Prion protein PrP antibody [F89/160.1.5]

• 产品名称

  Anti-Prion蛋白PrP抗体[F89/160.1.5]
  参阅全部 Prion protein PrP 一抗

• 描述

  小鼠单克隆抗体[F89/160.1.5] to Prion蛋白PrP

• 宿主

  Mouse

• 经测试应用

  适用于: Flow Cytmore details

• 种属反应性

  与反应: Human
  预测可用于: Pig

• 免疫原

  Synthetic peptide corresponding to Cow Prion protein PrP aa 146-159. U.S. Patent Number: 4,806,627.
  Sequence:

  SRPLIHFGSDYEDR

  
  (Peptide available as ab4908)

  Run BLAST with Run BLAST with 

• 表位

  This antibody is known to specifically recognize a conserved epitope of the PrP(Sc) protein comprising the amino acids n-IHFG-c.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.05% Sodium azide
  Constituent: 0.1% BSA

• 浓度

• 200 µg 浓度为 1 mg/ml

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  F89/160.1.5

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab2777于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 5621 Human

• Entrez Gene: 494014 Pig

• Omim: 176640 Human

• SwissProt: P04156 Human

• Unigene: 472010 Human

• Unigene: 610285 Human

• Unigene: 727471 Human

• 别名

• CD230 antibody

• CD230 antigen antibody

• CJD antibody

• GSS antibody

• KURU antibody

• Major prion protein antibody

• p27 30 antibody

• PRIO_HUMAN antibody

• Prion protein antibody

• Prion related protein antibody

• PRIP antibody

• PRNP antibody

• PrP antibody

• PrP27 30 antibody

• PrP27-30 antibody

• PrP33-35C antibody

• PrPC antibody

• PrPSc antibody

• Sinc antibody

• Alternative prion protein; major prion protein antibody

• AltPrP antibody

• ASCR antibody

• 

  Flow Cytometry - Anti-Prion protein PrP antibody [F89/160.1.5] (ab2777)

  Overlay histogram showing SH-SH5Y cells stained with ab2777 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2777, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.