Anti-beta Actin antibody [AC-15]

• 产品名称

  Anti-beta Actin抗体[AC-15]
  参阅全部 beta Actin 一抗

• 描述

  小鼠单克隆抗体[AC-15] to beta Actin

• 宿主

  Mouse

• 经测试应用

  适用于: ICC/IF, WBmore details

• 种属反应性

  与反应: Mouse, Rat, Cow, Dog, Human, African green monkey, Chinese hamster
  不与反应: Drosophila melanogaster, Dictyostelium discoideum

• 免疫原

  Synthetic peptide corresponding to beta Actin aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin. Slightly modified ß-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly -Ser-Gly-Lys, conjugated to KLH.
  Database link: P60709

  Run BLAST with Run BLAST with 

• 表位

  N-terminal of the beta isoform of actin.

• 阳性对照

• ICC/IF: SV40LT-SMC cells WB: HAP1, HeLa, Jurkat, A431, HEK-293, COS-7, NIH/3T3, PC-12, Rat2, CHO, MDBK and MDCK cell lysates.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.097% Sodium azide
  Constituent: PBS

• 浓度

• 批次浓度范围 50 µl 浓度为 2.1 - 2.3 mg/ml

• 纯度

  Affinity purified

• 纯化说明

  Purified from hybridoma cell culture.

• 克隆

  单克隆

• 克隆编号

  AC-15

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab6276于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 280979 Cow

• Entrez Gene: 403580 Dog

• Entrez Gene: 60 Human

• Entrez Gene: 11461 Mouse

• Entrez Gene: 81822 Rat

• Omim: 102630 Human

• SwissProt: P48975 Chinese hamster

• SwissProt: P60712 Cow

• SwissProt: O18840 Dog

• SwissProt: P60709 Human

• SwissProt: P60710 Mouse

• SwissProt: P60711 Rat

• Unigene: 520640 Human

• Unigene: 708120 Human

• Unigene: 727576 Human

• Unigene: 328431 Mouse

• Unigene: 391967 Mouse

• Unigene: 94978 Rat

别名

• A26C1A antibody

• A26C1B antibody

• ACTB antibody

• ACTB_HUMAN antibody

• Actin beta antibody

• Actin cytoplasmic 1 antibody

• Actin, cytoplasmic 1, N-terminally processed antibody

• Actx antibody

• b actin antibody

• b-actin antibody

• Beta cytoskeletal actin antibody

• Beta-actin antibody

• BRWS1 antibody

• E430023M04Rik antibody

• Melanoma X actin antibody

• MGC128179 antibody

• PS1TP5 binding protein 1 antibody

• PS1TP5BP1 antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [AC-15] (ab6276)

  ab6276 staining beta Actin in SV40LT-SMC cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab6276 at a working concentration of 5μg/ml and ab190573, Rabbit monoclonal [EP1332Y] to alpha Tubulin (Alexa Fluor® 647, shown in red) at 1/250 overnight at +4°C, followed by a further incubation at room temperature for 1h with an anti-mouse AlexaFluor® 488 (ab150117) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• 

  Western blot - Anti-beta Actin antibody [AC-15] (ab6276)

  All lanes : Anti-beta Actin antibody [AC-15] (ab6276) at 1/5000 dilution
  
  Lane 1 : Wild-type HAP1 cell lysate (20 µg)
  Lane 2 : Beta actin knockout HAP1 cell lysate (20 µg)
  
  Secondary
  All lanes : Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) at 1/10000 dilution
  
  Predicted band size: 42 kDa
  
  
  
  

  Lane 1: Wild-type HAP1 cell lysate (20 µg)
  Lane 2: Beta actin knockout HAP1 cell lysate (20 µg)

  Lanes 1 and 2: Merged signal (red and green). Green - beta actin, ab6276 observed at 42 kDa. Red - loading control, ab181602 observed at 37 kDa.

  Ab6276 was shown to specifically react with beta actin in wild-type HAP1 cells. No band was observed when beta actin knockout samples were used. Wild-type and beta actin knockout samples were subjected to SDS-PAGE. ab6276 (beta actin) and ab181602 (loading control to GAPDH) were diluted 1/5000 and 1/10 000 and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

• 

  Western blot - Anti-beta Actin antibody [AC-15] (ab6276)This image is courtesy of an Abreview submitted by Dr Mark Elliott

  Western Blot of ab6276 (used as loading control) with whole tissue lysate of human grey matter from BA20 (temporal cortex).  Ab6276 was diluted 1/50000 and incubated with the sample for 16 hours at 4°C.  5 µg of lysate was loaded onto the gel, which was blocked with 5% milk for 1 hour at 20°C.  An Alexa Fluor® 680 conjugated goat anti-mouse antibody, diluted 1/5000, was used as the secondary.

  Bands below actin in image are synaptophysin (SYN).

  See Abreview

• 

  Western blot - Anti-beta Actin antibody [AC-15] (ab6276)

  All lanes : Anti-beta Actin antibody [AC-15] (ab6276) at 1 µg/ml
  
  Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
  Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
  Lane 3 : COS-7 (african green monkey kidney fibroblast-like cell line) cell lysate
  Lane 4 : NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate
  Lane 5 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate
  Lane 6 : Rat2 (rat fibroblast cell line) cell lysate
  Lane 7 : CHO (chinese hamster ovary cell line) cell lysate
  Lane 8 : MDBK (bovine kidney cell line) cell lysate
  Lane 9 : MDCK (canine kidney cell line) cell lysate
  
  Secondary
  All lanes : Goat Anti-Mouse IgG-Peroxidase
  
  Developed using the ECL technique.
  
  Predicted band size: 42 kDa
  
  

• 

  Western blot - Anti-beta Actin antibody [AC-15] (ab6276)

  All lanes : Anti-beta Actin antibody [AC-15] (ab6276) at 1/5000 dilution
  
  Lane 1 : HeLa nuclear
  Lane 2 : HeLa whole cell lysate
  Lane 3 : A431 cell lysate
  Lane 4 : Jurkat cell lysate
  Lane 5 : HEK293 cell lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  All lanes : Alexa Fluor anti mouse at 1/5000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size: 42 kDa
  Observed band size: 42 kDa
  
  

• 

  Western blot - Anti-beta Actin antibody [AC-15] (ab6276)This image is courtesy of David Grimm, Yale University, USA.

  MDCK cells induced with increasing amounts of doxycycline to control expression of the gene of interest. All cells were normalized for loading with an albumin protein standard assay. Anti-beta actin (ab6276) was used at a concentration of 1:5000 in a milk blocking solution. B-actin blotting confirms the albumin assay in showing that an equal amount of lysate was loaded in each lane.