Anti-alpha Adaptin antibody [AC1-M11]

• 产品名称

  Anti-alpha Adaptin抗体[AC1-M11]
  参阅全部 alpha Adaptin 一抗

• 描述

  小鼠单克隆抗体[AC1-M11] to alpha Adaptin

• 宿主

  Mouse

• 经测试应用

  适用于: WB, ICC/IF, IHC-P, Flow Cytmore details

• 种属反应性

  与反应: Mouse, Rat, Human
  

• 免疫原

  Full length native protein (purified) corresponding to Cow alpha Adaptin. Purified Bovine brain adaptor complexes

• 阳性对照

• pig brain lysate and transfected COS cells.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.05% Sodium azide
  Constituent: PBS

• 浓度

• 500 µl 浓度为 2 mg/ml

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  AC1-M11

• 同种型

  IgG2a

The Abpromise guarantee

Abpromise™承诺保证使用ab2807于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 160 Human

• Entrez Gene: 161 Human

• Entrez Gene: 11771 Mouse

• Entrez Gene: 11772 Mouse

• Entrez Gene: 308578 Rat

• Entrez Gene: 81637 Rat

• Omim: 601026 Human

• Omim: 607242 Human

• SwissProt: O94973 Human

• SwissProt: O95782 Human

• SwissProt: P17426 Mouse

• SwissProt: P17427 Mouse

• SwissProt: P18484 Rat

别名

• 100 kDa coated vesicle protein A antibody

• 100 kDa coated vesicle protein C antibody

• Adapter related protein complex 2 subunit alpha 1 antibody

• Adapter related protein complex 2 subunit alpha 2 antibody

• Adaptin alpha A antibody

• Adaptin alpha B antibody

• Adaptin, alpha B antibody

• Adaptor protein complex AP 2 alpha 1 subunit antibody

• Adaptor protein complex AP 2 alpha 2 subunit antibody

• Adaptor protein complex AP 2 subunit alpha 1 antibody

• Adaptor related protein complex 2 alpha 1 subunit antibody

• Adaptor related protein complex 2 alpha 2 subunit antibody

• ADTAA antibody

• ADTAB antibody

• Alpha adaptin A antibody

• Alpha adaptin C antibody

• Alpha adaptin C; Huntingtin interacting protein J antibody

• Alpha1 adaptin antibody

• Alpha2 adaptin antibody

• AP 2 complex subunit alpha 1 antibody

• AP 2 complex subunit alpha 2 antibody

• AP2 alpha antibody

• AP2A1 antibody

• AP2A2 antibody

• CLAPA1 antibody

• CLAPA2 antibody

• Clathrin assembly protein complex 2 alpha A large chain antibody

• Clathrin assembly protein complex 2 alpha C large chain antibody

• Clathrin associated/assembly/adaptor protein large alpha 1 antibody

• Clathrin associated/assembly/adaptor protein large alpha 2 antibody

• HIP 9 antibody

• HIP9 antibody

• Huntingtin interacting protein antibody

• Huntingtin interacting protein HYPJ antibody

• Huntingtin interacting protein J antibody

• Huntingtin yeast partner J antibody

• HYPJ antibody

• Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit antibody

• Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit antibody

• 

  Western blot - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)

  All lanes : Anti-alpha Adaptin antibody [AC1-M11] (ab2807) at 2 µg/ml
  
  Lane 1 : Rat brain tissue lysate
  Lane 2 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
  Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
  Lane 4 : COLO 205 (Human colon adenocarcinoma cell line) whole cell lysate
  Lane 5 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate
  Lane 6 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
  Lane 7 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
  Lane 8 : A-431 (Human epidermoid carcinoma cell line) whole cell lysate
  
  Lysates/proteins at 30 µg per lane.
  
  Secondary
  All lanes : Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/2500 dilution
  
  Predicted band size: 108 kDa
  
  
  
  

  Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel, XCell SureLock™ Electrophoresis System and Novex® Sharp Pre-Stained Protein Standard. Resolved proteins were then transferred onto a nitrocellulose membrane with Pierce™ Power Blotter System (22834). The membrane was probed with the relevant primary and secondary Antibody using iBind™ Flex Western Starter Kit. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)

  Immunohistochemistry was performed on normal biopsies of deparaffinized Human heart tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer and microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a Mouse monoclonal antibody recognizing alpha Adaptin (ab2807) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)

  Immunolocalization of alpha-adaptin in NRK cells using ab2807.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)

  Immunolocalization of alpha-adaptin in NRK cells using ab2807 (low power image of image 2).

• 

  Flow Cytometry - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)

  Overlay histogram showing HepG2 cells stained with ab2807 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2807, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.