Anti-Transferrin Receptor antibody [OX26]

• 产品名称

  Anti-Transferrin Receptor抗体[OX26]
  参阅全部 Transferrin Receptor 一抗

• 描述

  小鼠单克隆抗体[OX26] to Transferrin Receptor

• 宿主

  Mouse

• 经测试应用

  适用于: Flow Cyt, IHC-Pmore details

• 种属反应性

  与反应: Mouse, Rat
  

• 免疫原

  Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

• 阳性对照

• Flow Cyt: Lewis rat bone marrow cells. IHC-P: Mouse and rat placenta tissue.

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.02% Sodium azide
  Constituent: PBS

• 浓度

• 10 µg 浓度为 1 mg/ml

• 100 µg 浓度为 1 mg/ml

• 纯度

  Affinity purified

• 克隆

  单克隆

• 克隆编号

  OX26

• 同种型

  IgG2a

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab6331于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 22042 Mouse

• Entrez Gene: 64678 Rat

• SwissProt: Q62351 Mouse

• SwissProt: Q99376 Rat

• 别名

• IMD46 antibody

• OTTHUMP00000208523 antibody

• OTTHUMP00000208524 antibody

• OTTHUMP00000208525 antibody

• p90 antibody

• sTfR antibody

• T9 antibody

• TFR 1 antibody

• TfR antibody

• TfR1 antibody

• TFR1_HUMAN antibody

• TFRC antibody

• TR antibody

• Transferrin receptor (p90 CD71) antibody

• Transferrin receptor protein 1, serum form antibody

• Trfr antibody

• CD 71 antibody

• CD71 antibody

• CD71 antigen antibody

• 

  Flow Cytometry - Anti-Transferrin Receptor antibody [OX26] (ab6331)

  Lewis rat bone marrow cells stained with ab6331 (right) or mouse IgG2a kappa (ab170191) isotype (left). Cells were incubated for 30 min on ice in 1x PBS containing 10 % rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab6331) or mouse IgG2a kappa (ab170191) isotype (1x 106 in 100 µl at 0.2 µg/ml) for 30 min on ice.

  The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor ®488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min on ice.

  The cells were simultaneously stained with Ter-119.

  Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on live single cells.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [OX26] (ab6331)

  IHC image of Transferrin receptor staining in a section of formalin-fixed paraffin-embedded normal rat placenta performed on a Leica BOND™ system using the standard Protocol F, omitting the post-primary step. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH9, epitope retrieval solution 2) for 20mins. The section was then incubated with ab6331, 5µg/ml, for 15 mins at room temperature. The section was then incubated with ab98792 (Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L, pre-adsorbed) 1/500 for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [OX26] (ab6331)

  IHC image of Transferrin receptor staining in normal mouse placenta formalin fixed paraffin embedded tissue section, using Mouse on Mouse detection kit ab127055. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab6331, 5µg/ml overnight at +4°C. The section was counterstained with hematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.