Anti-IL-2 Receptor alpha抗体[OX39]
参阅全部 IL-2 Receptor alpha 一抗
小鼠单克隆抗体[OX39] to IL-2 Receptor alpha
Mouse
适用于: IHC-Fr, Flow Cytmore details
与反应: Rat, Human
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
Flow Cyt: Lewis rat splenocytes treated with 5 g/ml ConA for 3 days. IHC-Fr: Normal rat spleen tissue.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
pH: 7.4
Preservative: 0.01% Sodium azide
Constituent: PBS
浓度
10 µg 浓度为 1 mg/ml
100 µg 浓度为 1 mg/ml
Affinity purified
单克隆
OX39
IgG1
kappa
Abpromise™承诺保证使用ab6411于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr | Use a concentration of 5 µg/ml. | |
| Flow Cyt | Use a concentration of 0.2 µg/ml. |
Entrez Gene: 3559 Human
Omim: 147730 Human
SwissProt: P01589 Human
Unigene: 231367 Human
Unigene: 9872 Rat
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![Flow Cytometry - Anti-IL-2 Receptor alpha antibody [OX39] (ab6411)](https://www.abcam.cn/ps/products/6/ab6411/Images/ab6411-355366-antiratIl2receptoralphaCD25Flowcytometryactivatedsplenocytesspleen.jpg)
Flow Cytometry - Anti-IL-2 Receptor alpha antibody [OX39] (ab6411)
Lewis rat splenocytes (top) or Lewis rat splenocytes treated with 5 µg/ml ConA for 3 days (bottom) were stained with ab6411 (right) or mouse IgG1 kappa (ab170190) isotype (left).
Splenocytes were incubated for 30 min on ice in 1x PBS containing 10 % rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab6411) or mouse IgG1 kappa (ab170190) isotype (1x 106 in 100 µl at 0.2 µg/ml) for 30 min on ice.
The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min on ice.
The cells were simultaneously stained with CD4.
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on CD3 positive T cells.
![Immunohistochemistry (Frozen sections) - Anti-IL-2 Receptor alpha antibody [OX39] (ab6411)](https://www.abcam.cn/ps/products/6/ab6411/Images/ab6411-355343-anti-il2ra-ox39-ihcfr-rt-spleen.jpg)
Immunohistochemistry (Frozen sections) - Anti-IL-2 Receptor alpha antibody [OX39] (ab6411)
IHC image of IL2 receptor alpha staining in a section of frozen normal rat spleen.
The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab6411 at 5µg/ml and ab16669 (Rabbit monoclonal [SP7] to CD3) at 1/150. The section was then incubated with ab150117 (Goat Anti-Mouse IgG H&L (Alexa Fluor®488), 1/1000)) (shown in green) and ab150080 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594), 1/1000) (shown in red) for 1 hour at room temperature. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. The secondary-only control insert image is taken from an identical assay without primary antibody. The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.
