Anti-Menin抗体
参阅全部 Menin 一抗
兔多克隆抗体to Menin
Rabbit
适用于: ICC/IF, ChIP, IHC-P, WB, IPmore details
与反应: Mouse, Human
预测可用于: Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Baboon, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to Human Menin.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
浓度
50 µg 浓度为 1 mg/ml
Affinity purified using the immunising peptideimmobilized on solid support.
多克隆
IgG
Abpromise™承诺保证使用ab2605于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF | (1) | Use at an assay dependent concentration. |
ChIP | Use at an assay dependent concentration. | |
IHC-P | 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
WB | 1/10000 - 1/25000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). | |
IP | Use at 2-10 µg/mg of lysate. |
Entrez Gene: 100722977 Guinea pig
Entrez Gene: 100050500 Horse
Entrez Gene: 4221 Human
Entrez Gene: 17283 Mouse
Omim: 613733 Human
SwissProt: O00255 Human
SwissProt: O88559 Mouse
Unigene: 423348 Human
Unigene: 12917 Mouse
Unigene: 453222 Mouse
Unigene: 6775 Rat
MEA 1 antibody
MEA1 antibody
MEN 1 antibody
Men1 antibody
MEN1_HUMAN antibody
Menin antibody
Multiple Endocrine Adenomatosis 1 antibody
Multiple Endocrine Neoplasia 1 antibody
SCG 2 antibody
SCG2 antibody
Suppressor Candidate Gene 2 antibody
Wermer syndrome antibody
ZES antibody
Zollinger Ellison Syndrome antibody
Western blot - Anti-Menin antibody - ChIP Grade (ab2605)
All lanes : Anti-Menin antibody (ab2605) at 0.04 µg/ml
Lane 1 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 68 kDa
Samples in NETN lysis buffer.
Exposure time: 30 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody - ChIP Grade (ab2605)
Immunohistochemical analysis of human lung carcinoma tissue labeling Menin with ab2605 at 1 µg/ml. Detection: DAB.
Immunoprecipitation - Anti-Menin antibody (ab2605)
Menin was imunnoprecipitated from 1.0 mg of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab2605 at 6 µg/ml. Western blot was perfomed from the immunoprecipitate using ab2506 at 0.04 µg/ml.
Lane 1 and 2: 20% of ab2605 IP in HEK-293T whole cell lysate
Lane 3: Control IgG
Exposure time: 1 second
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody - ChIP Grade (ab2605)
Immunohistochemical analysis of human breast carcinoma tissue labelling Menin with ab2605 at 1µg/ml. Detection: DAB.