Anti-Calcium Pump PMCA3 ATPase抗体
参阅全部 Calcium Pump PMCA3 ATPase 一抗
兔多克隆抗体to Calcium Pump PMCA3 ATPase
Rabbit
Detects PMCA 3a and 3b ATPase.
适用于: IHC, IHC-Pmore details
与反应: Human
Synthetic peptide corresponding to Rat Calcium Pump PMCA3 ATPase aa 1-100.
IHC: Human skeletal muscle tissue ; Human brain tissue ; Liver tissue
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab3530于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC | 1/20. | |
| IHC-P | (1) | 1/20. |
Entrez Gene: 492 Human
Omim: 300014 Human
SwissProt: Q16720 Human
ATPase plasma membrane Ca2+ transporting 3 antibody
Plasma membrane calcium ATPase 3 antibody
Plasma membrane calcium ATPase isoform 3 antibody
Plasma membrane calcium pump isoform 3 antibody
Plasma membrane calcium transporting ATPase 3 antibody
PMCA 3 antibody
PMCA3 antibody
PMCA3a antibody
ATP2B3 antibody
ATP2B3 protein antibody
ATPase Ca++ transporting plasma membrane 3 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA3 ATPase antibody (ab3530)
Immunohistochemistry was performed on normal biopsies of deparaffinized Human skeletal muscle tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing PMCA3 ATPase ab3530 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA3 ATPase antibody (ab3530)
Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing PMCA3 ATPase ab3530 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Immunohistochemistry - Anti-Calcium Pump PMCA3 ATPase antibody (ab3530)
Immunohistochemical analysis of liver and liver cancer tissue labelling Calcium Pump PMCA3 ATPase with ab3530. No staining observed in secondary antibody only control, and human tonsil which does not express Calcium Pump PMCA3 ATPase.
