Anti-Cyclophilin 40抗体
参阅全部 Cyclophilin 40 一抗
兔多克隆抗体to Cyclophilin 40
Rabbit
Detects cyclophilin 40 (CyP 40) from Human and Rat tissues and cells. This antibody does not cross-react with CyPA.
适用于: WB, IHC-P, ICC/IFmore details
与反应: Rat, Human
Synthetic peptide corresponding to Human Cyclophilin 40 aa 356-370.
Sequence:
AQKDKEKAVYAKMFA
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituent: 99% PBS
浓度
100 µl 浓度为 1 mg/ml
Proprietary Purification
Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents: cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins. CyP 40, a 40 kDa protein, shares significant homology with smaller CyPA (CyP 18) and FKBP59. CyP 40 exhibits the characteristic CsA binding and isomerase activity of CyP 18, though these activities appear to be less with CyP 40 than with Cyp 18. Like FKBP59, CyP 40 has been found in progesterone receptor complexes. CyP 40 is expressed at similar levels in many tissues.
多克隆
IgG
Abpromise™承诺保证使用ab3562于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/1000. | |
| IHC-P | Use at an assay dependent concentration. | |
| ICC/IF | 1/200. |
Entrez Gene: 5481 Human
Omim: 601753 Human
SwissProt: Q08752 Human
Unigene: 183958 Human
Unigene: 136915 Rat
Cyclophilin D antibody
Cyclophilin related protein antibody
Cyclophilin-40 antibody
Cyclophilin-related protein antibody
Cyclophilin40 antibody
CyclophilinD antibody
CYP 40 antibody
Cyp D antibody
CYP-40 antibody
CYP40 antibody
CYPD antibody
MGC33096 antibody
Peptidyl Prolyl Isomerase D antibody
Peptidyl-prolyl cis-trans isomerase D antibody
Peptidylprolyl isomerase D antibody
PPIase antibody
PPIase D antibody
Ppid antibody
PPID_HUMAN antibody
Rotamase antibody
Rotamase D antibody
40 kDa peptidyl prolyl cis trans isomerase antibody
40 kDa peptidyl prolyl cis trans isomerase D antibody
40 kDa peptidyl-prolyl cis-trans isomerase antibody
Western blot - Anti-Cyclophilin 40 antibody (ab3562)
ab3562 at a dilution of 1/1000 staining Cyp 40 in Rat spleen lysate by Western blot.
Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin 40 antibody (ab3562)
Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labeling Cyclophilin 40 (green) with ab3562 at 1/200. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.
Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin 40 antibody (ab3562)
Immunocytochemistry/Immunofluorescence analysis of A431 cells labeling Cyclophilin 40 (green) with ab3562 at 1/200. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin 40 antibody (ab3562)
Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human hepatocarcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at aab3562) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin 40 antibody (ab3562)
Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human skeletal muscle tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1/100 with a rabbit polyclonal antibody recognizing Cyclophilin D (ab3562) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
