Anti-Pumilio 1 antibody

• 产品名称

  Anti-Pumilio 1抗体
  参阅全部 Pumilio 1 一抗

• 描述

  山羊多克隆抗体to Pumilio 1

• 宿主

  Goat

• 经测试应用

  适用于: IP, WBmore details

• 种属反应性

  与反应: Mouse, Human
  预测可用于: Rat, Rabbit, Horse, Cow, Pig, Chimpanzee, Drosophila melanogaster, Rhesus monkey, Gorilla, Orangutan, Elephant

• 免疫原

  Synthetic peptide (Human) - which represented a portion of human Pumilio homolog 1 encoded in part by exons 5 and 6.

• 阳性对照

• WB: NIH/3T3, HEK293T, A549, K562 whole cell lysate; IP: HEK293T

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液

  pH: 7
  Preservative: 0.1% Sodium azide
  Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris

• 浓度

• 50 µl 浓度为 1 mg/ml

• 纯度

  Immunogen affinity purified

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab3717于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

  hide

• Unigene: 281707 Human

• Unigene: 440206 Mouse

• Entrez Gene: 535450 Cow

• Entrez Gene: 9698 Human

• Entrez Gene: 80912 Mouse

• Entrez Gene: 362609 Rat

• Omim: 607204 Human

• SwissProt: Q14671 Human

• SwissProt: Q80U78 Mouse

• SwissProt: Q5R5X3 Orangutan

别名

• HsPUM antibody

• KIAA0099 antibody

• PUM 1 antibody

• PUM L1 antibody

• Pum1 antibody

• PUM1_HUMAN antibody

• PUMH 1 antibody

• PUMH antibody

• PUMH1 antibody

• pumilio homolog 1 (Drosophila) antibody

• Pumilio homolog 1 antibody

• Pumilio-1 antibody

• Pumilio1 antibody

• PUML 1 antibody

• PUML1 antibody

• 

  Immunoprecipitation - Anti-Pumilio 1 antibody (ab3717)

  Pumilio 1 was immunoprecipitated from HEK293T whole cell lysate (1 mg per IP reaction, 20% loaded) with ab3717 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab3717 at 0.1 µg/mL.

  Lane 1: rabbit anti-Pumillio 1 antibody IP in HEK293T whole cell lysate.

  Lane 2: ab3717 IP in HEK293T whole cell lysate.

  Lane 3: ab3717 IP in HEK293T whole cell lysate

  Lane 4: Control IgG in HEK293T whole cell lysate.

  Detection: Chemiluminescence with an exposure time of 3 seconds

• 

  Western blot - Anti-Pumilio 1 antibody (ab3717)

  All lanes : Anti-Pumilio 1 antibody (ab3717) at 0.1 µg/ml
  
  Lane 1 : HEK293T whole cell lysate
  Lane 2 : A549 whole cell lysate
  Lane 3 : K562 whole cell lysate
  
  Lysates/proteins at 50 µg per lane.
  
  Secondary
  All lanes : HRP-conjugated rabbit anti-goat IgG
  
  Predicted band size: 126 kDa
  
  
  Exposure time: 10 seconds
  

• 

  Western blot - Anti-Pumilio 1 antibody (ab3717)

  Anti-Pumilio 1 antibody (ab3717) at 0.1 µg/ml + NIH/3T3 whole cell lysate at 50 µg
  
  Secondary
  HRP-conjugated rabbit anti-goat IgG
  
  Predicted band size: 126 kDa
  
  
  Exposure time: 10 seconds