Anti-PC1/3抗体
参阅全部 PC1/3 一抗
兔多克隆抗体to PC1/3
Rabbit
适用于: WBmore details
与反应: Mouse
预测可用于: Rat, Cow, Human
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
浓度
100 µg 浓度为 1 mg/ml
Immunogen affinity purified
The subtilisin-like Prohormone Convertase (PC) family is a group of cellular enzymes that cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. PC family members share structural similarities, which include a heterogeneous ~10 kDa amino-terminal proregion, a highly conserved ~55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. The subcellular localization of PC family members varies. Immunolocalization studies show that PC1 is found in the perinuclear region as well as the trans-Golgi network, whereas PC2 can be found in the trans-Golgi network as well as diffusely distributed in the peripheral cytoplasm.
多克隆
IgG
Abpromise™承诺保证使用ab3532于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 0.4 µg/ml. Detects a band of approximately 87 kDa (predicted molecular weight: 80 kDa). |
Entrez Gene: 5122 Human
Entrez Gene: 18548 Mouse
Omim: 162150 Human
SwissProt: P29120 Human
SwissProt: P63239 Mouse
Unigene: 78977 Human
Unigene: 1333 Mouse
Unigene: 11384 Rat
BDP antibody
NEC 1 antibody
NEC1 antibody
NEC1_HUMAN antibody
Neuroendocrine convertase 1 antibody
PC1 antibody
PC3 antibody
PCSK1 antibody
Prohormone convertase 1 antibody
Prohormone convertase 1/3 antibody
Prohormone convertase 3 antibody
Proprotein convertase 1 antibody
SPC3 antibody
Western blot - Anti-PC1/3 antibody (ab3532)
Western blot detection of PC1/3 from mouse pituitary extract using ab3532.
Western blot - Anti-PC1/3 antibody (ab3532)
Anti-PC1/3 antibody (ab3532) at 1 µg/ml + Spinal Cord (Mouse) Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 80 kDa
Observed band size: 68-87 kDawhy is the actual band size different from the predicted?
Additional bands at: 35 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
PC1/3 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. The band observed at 68 kDa could potentially be a cleaved form of PC1/3 due to the presence of a 27 amino acid signal peptide and an 83 amino acid propeptide.
