Anti-Cyclin B1 (phospho S126)抗体
参阅全部 Cyclin B1 一抗
兔多克隆抗体to Cyclin B1 (phospho S126)
Rabbit
This antibody is specific for phosphorylated human Cyclin B1 protein at the pS126 residue. BLAST analysis indicates 100 % homology of the immunizing sequence with Cyclin B1 from human. Cross reactivity may occur with Cyclin B1 from rat, dog and chimpanzee as only a single amino acid change was detected within the immunogen sequence from these sources (91% homology). Partial reactivity may also occur with Cyclin B1 from mouse and hamster where two amino acids changes was noted (83% homology). Minimal reactivity is expected with the non-phosphorylated form of the protein.
适用于: WBmore details
与反应: Human
预测可用于: Mouse, Rat, Hamster, Dog, Chimpanzee
Synthetic peptide corresponding to Human Cyclin B1 aa 120-131 (phospho S126).
Sequence: ILVDTASpPSPME
Database link: P14635
This antibody recognizes a Ser126 phosphorylation involved in an auto phosphorylation of the cdk -cyclin complex. Recognition of this phosphorylated site suggests the antibody is useful to detect the active form of the complex and may serve as a marker of mitosis.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride
Immunogen affinity purified
This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.
This antibody recognizes a Ser126 phosphorylation involved in an auto phosphorylation of the cdk -cyclin complex. Recognition of this phosphorylated site suggests the antibody is useful to detect the active form of the complex and may serve as a marker of mitosis.
多克隆
IgG
Abpromise™承诺保证使用ab3488于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/100 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). |
Entrez Gene: 461835 Chimpanzee
Entrez Gene: 101836169 Hamster
Entrez Gene: 891 Human
Entrez Gene: 268697 Mouse
Omim: 123836 Human
SwissProt: P37882 Hamster
SwissProt: P14635 Human
SwissProt: P24860 Mouse
Unigene: 23960 Human
Unigene: 260114 Mouse
Unigene: 380027 Mouse
Unigene: 482545 Mouse
Unigene: 9232 Rat
CCNB 1 antibody
CCNB antibody
ccnb1 antibody
CCNB1_HUMAN antibody
Cyclin B1 antibody
G2 mitotic specific cyclin B1 antibody
G2/mitotic-specific cyclin-B1 antibody
Western blot - Anti-Cyclin B1 (phospho S126) antibody (ab3488)
All lanes : Anti-Cyclin B1 (phospho S126) antibody (ab3488) at 1/100 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : H23 whole cell lysate
Lane 3 : Hepatocarcinoma (Hep3b) cell lysate
Lane 4 : Glioblastoma (T98G) cell lysate
Lane 5 : B cell lymphoblast (Daudi) cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated Goat anti-Rabbit IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Exposure time: 20 seconds
