Anti-JunB抗体
参阅全部 JunB 一抗
兔多克隆抗体to JunB
Rabbit
适用于: WB, ICC/IFmore details
与反应: Mouse, Human
预测可用于: Rat, Cow
Synthetic peptide corresponding to Mouse JunB aa 1-100.
Database link: P09450
WB: uninduced and induced L929 cells. ICC/IF: PC-3 (Human prostate adenocarcinoma cell line)
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituent: 100% PBS
浓度
200 µg 浓度为 1 mg/ml
IgG fraction
多克隆
IgG
Abpromise™承诺保证使用ab3525于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 10 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa). | |
| ICC/IF | Use a concentration of 2 µg/ml. |
Entrez Gene: 3726 Human
Entrez Gene: 16477 Mouse
Omim: 165161 Human
SwissProt: P17275 Human
SwissProt: P09450 Mouse
Unigene: 25292 Human
Unigene: 1167 Mouse
Unigene: 470093 Mouse
Unigene: 15806 Rat
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Western blot - Anti-JunB antibody (ab3525)
All lanes : Anti-JunB antibody (ab3525)
Lane 1 : L929 cell extract
Lane 2 : Extract from L929 cells treated with dexamethasone
Predicted band size: 38 kDa
Observed band size: 38 kDa
Additional bands at: 50 kDa (possible cross reactivity)
ab3525 in Western blot on L929 cells. There is the specific band at 38 kDa. There are also non-specific bands at 50 kDa and a very faint one at 40 kDa.
Immunocytochemistry/ Immunofluorescence - Anti-JunB antibody (ab3525)
Immunofluorescent analysis of PC-3 (Human prostate adenocarcinoma cell line) cells on 70% confluent log phase labeling JunB. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ab3525 at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) secondary antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (1/300 dilution). Panel d is a merged image showing nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
