Anti-Calcium Pump PMCA2 ATPase antibody

• 产品名称

  Anti-Calcium Pump PMCA2 ATPase抗体
  参阅全部 Calcium Pump PMCA2 ATPase 一抗

• 描述

  兔多克隆抗体to Calcium Pump PMCA2 ATPase

• 宿主

  Rabbit

• 特异性

  Detects PMCA 2a and 2b ATPase.

• 经测试应用

  适用于: IHC-FoFr, ICC, ELISA, IHC-Fr, WB, ICC/IF, IHC-P, IPmore details

• 种属反应性

  与反应: Mouse, Rat, Chicken, Human, Pig, Non human primates
  

• 免疫原

  Synthetic peptide corresponding to Human Calcium Pump PMCA2 ATPase aa 1-100.

  Run BLAST with  Run BLAST with 

• 阳性对照

• IHC-P: Human brain and cervical carcinoma tissue.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.05% Sodium azide
  Constituents: 0.1% BSA, 99% PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Immunogen affinity purified

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab3529于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 491 Human

• Entrez Gene: 11941 Mouse

• Entrez Gene: 24215 Rat

• Omim: 108733 Human

• SwissProt: Q01814 Human

• SwissProt: Q9R0K7 Mouse

• SwissProt: P11506 Rat

• Unigene: 268942 Human

• Unigene: 321755 Mouse

• Unigene: 90982 Rat

别名

• AT2B2_HUMAN antibody

• atp2b2 antibody

• ATPase plasma membrane Ca2+ transporting 2 antibody

• Plasma membrane calcium ATPase 2 antibody

• Plasma membrane calcium ATPase antibody

• Plasma membrane calcium ATPase isoform 2 antibody

• Plasma membrane calcium pump isoform 2 antibody

• Plasma membrane calcium transporting ATPase 2 antibody

• Plasma membrane calcium-transporting ATPase 2 antibody

• PMCA2 antibody

• PMCA2a antibody

• PMCA2i antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA2 ATPase antibody (ab3529)

  Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a rabbit polyclonal antibody recognizing PMCA2 ATPase ab3529 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA2 ATPase antibody (ab3529)

  Human cerebellum (Centre panel) and lung (Negative control, right panel) stained for PMCA2 ATPase using ab3529 at 1/20 dilution in immunohistochemical analysis. The left hand panel is a secondary antibody only control.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA2 ATPase antibody (ab3529)

  Immunohistochemistry was performed on cancer biopsies of deparaffinized Human cervical carcinoma tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing PMCA2 ATPase ab3529 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.