Anti-Dnmt3L抗体
参阅全部 Dnmt3L 一抗
兔多克隆抗体to Dnmt3L
Rabbit
This antibody is expected to cross-react with mouse as the sequence of the human immunogen shows 76% identity with mouse.
适用于: IP, IHC-P, ELISA, WBmore details
与反应: Human
Synthetic peptide corresponding to Human Dnmt3L aa 152-164 conjugated to keyhole limpet haemocyanin.
Sequence:
C-GLLQRRRKWRSQL
Database link: Q9UJW3
DNMT 3L is a nuclear protein which has similarity to DNA methyltransferases, involved in de novo methylation of CpG islands. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride
浓度
100 µg 浓度为 1.11 mg/ml
Immunogen affinity purified
This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.
DNMT 3L is a nuclear protein which has similarity to DNA methyltransferases, involved in de novo methylation of CpG islands. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1.
多克隆
IgG
Abpromise™承诺保证使用ab3493于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | (6) | 1/100. |
| IHC-P | 1/500. | |
| ELISA | 1/1000 - 1/3000. | |
| WB | (8) | 1/500. Predicted molecular weight: 44 kDa. |
Entrez Gene: 29947 Human
Omim: 606588 Human
SwissProt: Q9UJW3 Human
Unigene: 592165 Human
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Immunoprecipitation - Anti-Dnmt3L antibody (ab3493)
Transfection of U2OS cells (10cm dish) using 3µg of GAL4-DNMT3L. Protein extraction using IPH 150mM. IP and WB conditions, see Fuks et al. (2000). Nature Genetics 24: 88-91.WB using 0.4µg/ml in TBS milk 2%, BSA 0.5%. Dectection using ECL.
Note: ab3493 worked in WB following IP, but did not work in a straight WB.
