Anti-IRAK4抗体
参阅全部 IRAK4 一抗
兔多克隆抗体to IRAK4
Rabbit
This antibody is specific to IRAK4 and does not cross react with other IRAK proteins.
适用于: ICC/IF, WBmore details
与反应: Human
Synthetic peptide corresponding to Human IRAK4 (C terminal).
(Peptide available as ab39782)
WB: Daudi, HepG2 and HeLa whole cell lysate. ICC/IF: K562 cells
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C. The product can be stored for up to 12 months.
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS
浓度
<div class="concentration-holder" data-hcaptchasitekey="193441f7-8cd9-40a4-8075-4640966eb995" "="" style="border: 1px solid rgb(222, 223, 223); font-family: inherit; font-style: inherit; font-weight: inherit; margin: 0px 0px 1em; outline: none 0px; padding: 10px; vertical-align: baseline; box-sizing: border-box; bottom: 5px; clear: both; width: 400px;">
100 µg 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab5985于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use a concentration of 10 µg/ml. | |
| WB | (1) | Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 50 kDa. |
Entrez Gene: 51135 Human
Omim: 606883 Human
SwissProt: Q9NWZ3 Human
Unigene: 138499 Human
Interleukin1 receptor associated kinase 4 antibody
IPD1 antibody
IRAK 4 antibody
IRAK-4 antibody
IRAK4 antibody
IRAK4 mutated form 1 antibody
IRAK4_HUMAN antibody
LOC 51135 antibody
NY REN 64 antibody
NY REN 64 antigen antibody
NY-REN-64 antibody
REN64 antibody
Renal carcinoma antigen NY-REN-64 antibody
IL-1 receptor-associated kinase 4 antibody
Interleukin 1 receptor associated kinase 4 mutant form 1 antibody
Interleukin-1 receptor-associated kinase 4 antibody
Western blot - Anti-IRAK4 antibody (ab5985)
All lanes : Anti-IRAK4 antibody (ab5985) at 1 µg/ml
Lane 1 : Daudi cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (ab5985)
Immunofluorescent analysis of 4% paraformaldehydefixed K562 Cells labeling IRAK4 with ab9585 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Western blot - Anti-IRAK4 antibody (ab5985)
Lane 1 : Anti-IRAK4 antibody (ab5985) at 1 µg/ml
Lane 2 : Anti-IRAK4 antibody (ab5985) at 2 µg/ml
Lane 3 : Anti-IRAK4 antibody (ab5985) at 4 µg/ml
All lanes : HeLa cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Primary incbation: 1 hour at room temperature in 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (ab5985)
Immunocytochemical analysis of K562 cells labeling IRAK4 with ab5985 at 10 μg/mL. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (ab5985)This image was kindly supplied by Dr Mahesh Shivananjappa by Abreview
ab5985 at a 1/100 dilution staining IRAK4 in human PMN cells by Immunocytochemistry/ Immunofluorescence incubated for 4 hours at 37°C. Fixed with PFA. Blocked using 2% BSA for 1 hour at 22°C. Secondary used at 1/250 polyclonal Goat anti-rabbit conjugated to Alexa Fluor 568.
Left image: DAPI staining nuclei (blue)
Middle image: IRAK4 (red)
Right image: Overlay
