Anti-RYBP antibody

• 产品名称

  Anti-RYBP抗体
  参阅全部 RYBP 一抗

• 描述

  兔多克隆抗体to RYBP

• 宿主

  Rabbit

• 经测试应用

  适用于: WB, IHC-P, ICC/IFmore details

• 种属反应性

  与反应: Mouse, Human
  

• 免疫原

  Synthetic peptide:

  TPKGDMSAVNDESF

  , corresponding to amino acids 215-228 of Human DEDAF.

  Run BLAST with Run BLAST with 

• 阳性对照

• A549, HepG2 or 3T3 whole cell lysate.

• 常规说明

  Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. Several molecules including caspases and adaptor FADD contain DEDs. A novel protein that interacts with DED of caspase 8 and 10, and FADD was identified recently and designated DEDAF for DED associated factor. DEDAF is identical to the transcriptional repressor RYBP. DEDAF/RYBP is expressed in multiple tissues and cell lines. DEDAF interacts with FADD and augments the formation of CD95/FADD/capase 8 complexes at the cell membrane, and interacts with DED-containing DNA biding protein (DEDD) in the nucleus indicating it is involved in the regulation of both cytoplasmic and nuclear events of apoptosis.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

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• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C.

• 存储溶液

  pH: 7.2
  Preservative: 0.02% Sodium azide

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Immunogen affinity purified

• 纯化说明

  DEDAF Antibody is affinity chromatography purified via peptide column.

• Primary antibody说明

  Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. Several molecules including caspases and adaptor FADD contain DEDs. A novel protein that interacts with DED of caspase 8 and 10, and FADD was identified recently and designated DEDAF for DED associated factor. DEDAF is identical to the transcriptional repressor RYBP. DEDAF/RYBP is expressed in multiple tissues and cell lines. DEDAF interacts with FADD and augments the formation of CD95/FADD/capase 8 complexes at the cell membrane, and interacts with DED-containing DNA biding protein (DEDD) in the nucleus indicating it is involved in the regulation of both cytoplasmic and nuclear events of apoptosis.

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab5976于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 23429 Human

• Entrez Gene: 56353 Mouse

• Omim: 607535 Human

• SwissProt: Q8N488 Human

• SwissProt: Q8CCI5 Mouse

• Unigene: 7910 Human

• Unigene: 321633 Mouse

• 别名

• APAP 1 antibody

• APAP-1 antibody

• APAP1 antibody

• Apoptin associating protein 1 antibody

• Apoptin-associating protein 1 antibody

• cb337 antibody

• Death effector domain associated factor antibody

• Death effector domain-associated factor antibody

• Death effector domain-associated factor B antibody

• DED associated factor antibody

• DED-associated factor antibody

• DED-associated factor B antibody

• DEDAF antibody

• MGC62213 antibody

• RING1 and YY1 binding protein a antibody

• RING1 and YY1 binding protein antibody

• RING1 and YY1-binding protein antibody

• RING1 and YY1-binding protein B antibody

• Ring1 interactor RYBP antibody

• RYBP antibody

• RYBP_HUMAN antibody

• rybpa antibody

• wu:fi34f03 antibody

• YEAF 1 antibody

• YEAF1 antibody

• YY1 and E4TF1 associated factor 1 antibody

• YY1 and E4TF1-associated factor 1 antibody

• YY1- and E4TF1/GABP-associated factor 1 antibody

• zgc:153842 antibody

• 2410018J24Rik antibody

• AAP 1 antibody

• AAP1 antibody

• 

  Western blot - Anti-RYBP antibody (ab5976)

  Western blot analysis of DEDAF expression in human A549 (lane A), HepG2 (lane B), and mouse 3T3 (lane C) cell lysates withab5976 at 1 µg /ml.

  Western blot analysis of DEDAF expression in human A549 (lane A), HepG2 (lane B), and mouse 3T3 (lane C) cell lysates withab5976 at 1 µg /ml.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RYBP antibody (ab5976)

  Immunohistochemistry of DEDAF in mouse liver tissue with DEDAF antibody at 5 µg/ml.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-RYBP antibody (ab5976)This image is courtesy of an Abreview submitted by Dr. Ligia Tavares

  ab5976 staining RYBP in Mouse embryonic stem cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton 0.5% and blocked with 1% milk for 30 minutes at 22°C. Samples were incubated with primary antibody (1/1000) for 1 hour at 22°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG (H+L) polyclonal (1/400) was used as the secondary antibody.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-RYBP antibody (ab5976)

  Immunofluorescence of DEDAF in A549 cells using ab5976 at 20 ug/ml.