Anti-Hsp90 antibody [AC88]

• 产品名称

  Anti-Hsp90抗体[AC88]
  参阅全部 Hsp90 一抗

• 描述

  小鼠单克隆抗体[AC88] to Hsp90

• 宿主

  Mouse

• 经测试应用

  适用于: Flow Cyt (Intra), IHC-P, ICC/IF, WBmore details

• 种属反应性

  与反应: Mouse, Rat, Human, Chinese hamster
  

• 免疫原

  Full length native protein (purified) corresponding to Hsp90. from Achlya ambisexualis (water mold).
  Database link: Q8LLI6

• 表位

  The epitope of this antibody has been mapped to amino acid residues 604-697 of the human Hsp90 sequence.

• 阳性对照

• ICC/IF: Panc-1 and HepG2 cells. WB: HeLa Cell Lysate (Heat Shocked), Hsp90 Protein; HepG2 cell lysate; PC12 cell lysate; NIH/3T3 cell lysate; Rat2 cell lysate. IHC-P: Human spleen and testis tissue. IHC-fr: Human stomach tissue.

• 常规说明

  This product was changed from ascites to tissue culture supernatant on 22nd May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.09% Sodium azide
  Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine)

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  AC88

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab13492于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 3320 Human

• Entrez Gene: 3326 Human

• Entrez Gene: 15516 Mouse

• Entrez Gene: 15519 Mouse

• Entrez Gene: 299331 Rat

• Entrez Gene: 301252 Rat

• Omim: 140571 Human

• Omim: 140572 Human

• SwissProt: P07900 Human

• SwissProt: P08238 Human

• SwissProt: P07901 Mouse

• SwissProt: P11499 Mouse

• SwissProt: P34058 Rat

• SwissProt: P82995 Rat

• Unigene: 525600 Human

• Unigene: 1843 Mouse

• Unigene: 315997 Mouse

• Unigene: 341186 Mouse

• Unigene: 440626 Mouse

• Unigene: 486334 Mouse

• Unigene: 119867 Rat

• Unigene: 232226 Rat

• Unigene: 3277 Rat

别名

• Heat shock 86 kDa antibody

• Heat shock protein 90kDa alpha cytosolic class A member 1 antibody

• Heat shock protein 90kDa alpha cytosolic class B member 1 antibody

• Heat shock protein HSP 90 alpha antibody

• Heat shock protein HSP 90 beta antibody

• Heat shock protein HSP 90-alpha antibody

• HS90A_HUMAN antibody

• HSP 84 antibody

• HSP 86 antibody

• Hsp 90 antibody

• HSP86 antibody

• HSP90A antibody

• HSP90AA1 antibody

• HSP90AB1 antibody

• HSP90B antibody

• HSPC1 antibody

• HSPC2 antibody

• HSPCAL1 antibody

• HSPCAL4 antibody

• Renal carcinoma antigen NY-REN-38 antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 antibody [AC88] (ab13492)This image is courtesy of an Abreview submitted by Armen Petrosyan

  ab13492 staining Hsp90 (red) and another antibody to C2GnT-M (Golgi enzyme, greeen) in Panc-1 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with PFA and blocked with 1% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/50 1% Donkey serum in PBST) for 1 hour at 22°C. An undiluted DyLight® 594-conjugated Donkey anti-mouse IgG polyclonal was used as the secondary antibody.

  This image was generated using the ascites version of the product. 

  See Abreview

• 

  Western blot - Anti-Hsp90 antibody [AC88] (ab13492)

  All lanes : Anti-Hsp90 antibody [AC88] (ab13492) at 1/1000 dilution
  
  Lane 2 : HSP90 cell lysate
  Lane 3 : HSP90ß cell lysate
  Lane 4 : HSP90a cell lysate
  Lane 5 : HeLa (heat shocked) cell lysate
  Lane 6 : NIH/3T3 (Heat shocked) cell lysate
  Lanes 7-8 : PC-12 (Heat shocked) cell lysate
  
  
  

  This image was generated using the ascites version of the product.

• 

  Western blot - Anti-Hsp90 antibody [AC88] (ab13492)This image is courtesy of an anonymous Abreview

  Anti-Hsp90 antibody [AC88] (ab13492) at 1/1000 dilution + Human fibroblast whole cell lysate at 40 µg
  
  Secondary
  HRP-conjugated goat anti-mouse polyclonal IgG at 1/2000 dilution
  
  Developed using the ECL technique.
  
  Exposure time: 20 seconds
  
  
  

  Blocked with 5% Milk for 2 hours at 22°C

  This image was generated using the ascites version of the product.

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 antibody [AC88] (ab13492)This image is courtesy of an Abreview submitted by Rudolf Jung

  ab13492 staining Hsp90 in Human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a TRIS-EDTA Buffer. Samples were incubated with primary antibody (1/500) for 30 minutes at 20°C. A HRP-conjugated Goat anti-rabbit/mouse IgG polyclonal was used as the secondary antibody.

  This image was generated using the ascites version of the product.

  See Abreview

• 

  Flow Cytometry (Intracellular) - Anti-Hsp90 antibody [AC88] (ab13492)

  Overlay histogram showing HeLa cells stained with ab13492 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum (ab7481) / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13492, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
  
  

  This image was generated using the ascites version of the product.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 antibody [AC88] (ab13492)

  ab13492 staining Hsp90 in Human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (10 ug/ml).

  This image was generated using the ascites version of the product.

• 

  Western blot - Anti-Hsp90 antibody [AC88] (ab13492)

  All lanes : Anti-Hsp90 antibody [AC88] (ab13492) at 1/1000 dilution
  
  Lane 1 : Hsp90 native human protein
  Lane 2 : Hsp90 beta reombinant human protein
  Lane 3 : Hsp90 alpha reombinant human protein
  Lane 4 : Cell lysates prepared from heat shocked Hela cells
  Lane 5 : Cell lysates prepared from heat shocked 3T3 cells
  Lane 6 : Cell lysates prepared from heat shocked PC-12 cells
  Lane 7 : Cell lysates prepared from heat shocked CHO-K1 cells
  Lane 8 : Cell lysates prepared from heat shocked Rat-2 cells
  
  
  

  This image was generated using the ascites version of the product.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 antibody [AC88] (ab13492)

  ICC/IF image of ab13492 stained HepG2 cells (ab7900). The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13492, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-mouse IgG - H&L, pre-adsorbed (ab96879) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  This image was generated using the ascites version of the product.