Anti-HDAC4抗体
参阅全部 HDAC4 一抗
兔多克隆抗体to HDAC4
Rabbit
适用于: ICC/IF, WB, IPmore details
与反应: Mouse, Rat, Human
预测可用于: Rabbit, Chicken
Synthetic peptide:
MSSQSHPDGLSGRDQPVEL
, corresponding to amino acids 1-19 of Human HDAC4 conjugated to KLH with C-terminal added lysine. The corresponding sequence is identical in mouse and chicken.
WB: NIH/3T3 whole cell extract, Rat brain cell extract. IP: HeLa whole cell extract. ICC/IF: HeLa tissue.
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 1% BSA
浓度
50 µl 浓度为 1.4 mg/ml
Immunogen affinity purified
The antibody is affinity-purified using the immunizing peptide immobilized on agarose.
多克隆
IgG
Abpromise™承诺保证使用ab12172于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (1) | 1/250 - 1/500. |
| WB | (5) | 1/500 - 1/2000. Predicted molecular weight: 119 kDa. Additional weak bands may be detected when blotting using different extract preparations. |
| IP | Use at an assay dependent concentration. 10-20 µg of antibody immunoprecipitates HDAC4 from a RIPA extract of HeLa nuclei from 1 x 107 cells. |
Entrez Gene: 9759 Human
Entrez Gene: 208727 Mouse
Omim: 605314 Human
SwissProt: P56524 Human
SwissProt: Q6NZM9 Mouse
Unigene: 20516 Human
Unigene: 318567 Mouse
Unigene: 23483 Rat
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Western blot - Anti-HDAC4 antibody (ab12172)
Lane 1 : Anti-HDAC4 antibody (ab12172) at 1/500 dilution
Lane 2 : Anti-HDAC4 antibody (ab12172) at 1/1000 dilution
Lanes 1-2 : Rat brain whole extract
Lane 3 : Negative control
Predicted band size: 119 kDa
Western blot - Anti-HDAC4 antibody (ab12172)
Lane 1 : Anti-HDAC4 antibody (ab12172) at 1/1000 dilution
Lane 2 : Anti-HDAC4 antibody (ab12172) at 1/2000 dilution
Lanes 1-2 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell extract
Lane 3 : Negative Control
Predicted band size: 119 kDa
Immunocytochemistry/ Immunofluorescence - Anti-HDAC4 antibody (ab12172)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Hela tissue labelling HDAC4 with ab12172 at 1/250 dilution.
Immunoprecipitation - Anti-HDAC4 antibody (ab12172)
Anti-HDAC4 antibody, ab12172, was used to immunoprecipitate HDAC4 from HeLa whole cell extract.
Lane 1: 10 µg of ab12172
Lane 2: 5 µg of ab12172
Negative control: No cell extract
Positive control: HeLa whole cell extract
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