Anti-Fos B antibody [83B1138]

• 产品名称

  Anti-Fos B抗体[83B1138]
  参阅全部 Fos B 一抗

• 描述

  小鼠单克隆抗体[83B1138] to Fos B

• 宿主

  Mouse

• 经测试应用

  适用于: IHC-Fr, Flow Cyt, WBmore details

• 种属反应性

  与反应: Mouse, Human
  

• 免疫原

  Synthetic peptide corresponding to Human Fos B.

• 阳性对照

• HeLa whole-cell extract.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• 存储溶液

  Preservative: 0.02% Sodium azide
  Constituent: PBS

• 浓度

• 50 µg 浓度为 1 mg/ml

• 纯度

  Protein G purified

• 纯化说明

  Affinity purified.

• 克隆

  单克隆

• 克隆编号

  83B1138

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab11959于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 2354 Human

• Entrez Gene: 14282 Mouse

• Omim: 164772 Human

• SwissProt: P53539 Human

• SwissProt: P13346 Mouse

• Unigene: 590958 Human

• Unigene: 248335 Mouse

• 别名

• FBJ murine osteosarcoma viral oncogene homolog B antibody

• fosB antibody

• FOSB_HUMAN antibody

• G0/G1 switch regulatory protein 3 antibody

• G0S3 antibody

• GOS 3 antibody

• GOS B antibody

• GOS3 antibody

• GOSB antibody

• MGC42291 antibody

• Oncogene FOS B antibody

• Oncogene FOSB antibody

• Protein fos B antibody

• Protein fosB antibody

• Activator protein 1 antibody

• AP 1 antibody

• DKFZp686C0818 antibody

• 

  Western blot - Anti-Fos B antibody [83B1138] (ab11959)

  ab11959 staining Human Fos B (upper band) in HeLa whole cell lysate by Western blot (ECL). The lower band is thought to be an isoform of FosB. ab11959 staining Human Fos B (upper band) in HeLa whole cell lysate by Western blot (ECL). The lower band is thought to be an isoform of FosB.

• 

  Flow Cytometry - Anti-Fos B antibody [83B1138] (ab11959)

  Overlay histogram showing HeLa cells stained with ab11959 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11959, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
  
  

• 

  Immunohistochemistry (Frozen sections) - Anti-Fos B antibody [83B1138] (ab11959)Image courtesy of Dr. Karine Thibault, Université Paris Descartes, France

  ab11959 Fos B antibody immunostaining (red) in mouse brain cortex showing the striatum. Tissue was fixed with paraformaldehyde, before incubation with the antibody (1/3000) for 18 hours at 25ºC. A donkey anti-mouse Alexa Fluor® 568 conjugated secondary was used at 1/1000.

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