Anti-Metallothionein antibody [UC1MT]

• 产品名称

  Anti-Metallothionein抗体[UC1MT]
  参阅全部 Metallothionein 一抗

• 描述

  小鼠单克隆抗体[UC1MT] to Metallothionein

• 宿主

  Mouse

• 经测试应用

  适用于: ICC/IF, WB, Flow Cytmore details

• 种属反应性

  与反应: Rabbit, Human
  

• 免疫原

  Full length protein corresponding to Rabbit Metallothionein. Cross-linked rabbit liver Metallothionein I and II.

• 阳性对照

• HeLa cell lysate treated with 100uM CdCl2 Rehydrated rabbit liver MTI/MTII

• 常规说明

  This product was changed from ascites to tissue culture supernatant on 22nd May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液

  Preservative: 0.09% Sodium azide
  Constituents: 2.68% PBS, 50% Glycerol (glycerin, glycerine)

• 纯度

  Tissue culture supernatant

• 纯化说明

  Purified from TCS.

• 克隆

  单克隆

• 克隆编号

  UC1MT

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab12228于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 4489 Human

• Entrez Gene: 4502 Human

• Omim: 156350 Human

• Omim: 156360 Human

• SwissProt: P02795 Human

• SwissProt: P04731 Human

• SwissProt: P11957 Rabbit

• SwissProt: P18055 Rabbit

• Unigene: 655199 Human

别名

• CES 1 antibody

• CES1 antibody

• Metallothionein 1A antibody

• Metallothionein 1S antibody

• Metallothionein 2 antibody

• Metallothionein 2A antibody

• Metallothionein IA antibody

• Metallothionein II antibody

• Metallothionein-1A antibody

• Metallothionein-IA antibody

• Metallothionein2 antibody

• MGC32848 antibody

• MT 1A antibody

• MT 2 antibody

• MT 2A antibody

• MT IA antibody

• MT II antibody

• MT-1A antibody

• MT-IA antibody

• MT1 antibody

• MT1A antibody

• MT1A_HUMAN antibody

• MT1S antibody

• MT2 antibody

• MT2A antibody

• MTC antibody

• 

  Western blot - Anti-Metallothionein antibody [UC1MT] (ab12228)

  Anti-Metallothionein antibody [UC1MT] (ab12228) + Hela cell lysate
  
  Secondary
  HRP-conjugated antibody.
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 6 kDa
  
  
  Exposure time: 2 minutes
  
  
  

  This image was generated using the ascites version of the product.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Metallothionein antibody [UC1MT] (ab12228)

  ICC/IF image of ab12228 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12228, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  This image was generated using the ascites version of the product.

• 

  Flow Cytometry - Anti-Metallothionein antibody [UC1MT] (ab12228)

  Overlay histogram showing HeLA cells stained with ab12228 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12228, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  This image was generated using the ascites version of the product.

• 

  Western blot - Anti-Metallothionein antibody [UC1MT] (ab12228)

  Anti-Metallothionein antibody [UC1MT] (ab12228) at 1/1000 dilution + Rabbit liver lysates
  
  Predicted band size: 6 kDa
  
  
  
  

  This image was generated using the ascites version of the product.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Metallothionein antibody [UC1MT] (ab12228)

  ICC/IF image of ab12228 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12228, 10µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-mouse IgG - H&L, pre-adsorbed (ab96879) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  This image was generated using the ascites version of the product.