Anti-NCAM1 antibody [ERIC-1]

• 产品名称

  Anti-NCAM1抗体[ERIC-1]
  参阅全部 NCAM1 一抗

• 描述

  小鼠单克隆抗体[ERIC-1] to NCAM1

• 宿主

  Mouse

• 经测试应用

  适用于: Flow Cyt, IHC-Pmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Tissue, cells or virus corresponding to NCAM1. Retinoblastoma tissue membrane fraction

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• 存储溶液

  Preservative: 0.02% Sodium azide
  Constituent: 99.98% PBS

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  ERIC-1

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab6123于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 4684 Human

• Omim: 116930 Human

• SwissProt: P13591 Human

• Unigene: 503878 Human

• 别名

• cell adhesion molecule, neural, 1 antibody

• MSK 39 antibody

• MSK39 antibody

• N-CAM-1 antibody

• NCAM 1 antibody

• NCAM antibody

• NCAM C antibody

• NCAM-1 antibody

• NCAM1 antibody

• NCAM1_HUMAN antibody

• NCAMC antibody

• Neural cell adhesion molecule 1 antibody

• Neural cell adhesion molecule NCAM antibody

• OTTHUMP00000235666 antibody

• antigen MSK39 identified by monoclonal antibody 5.1H11 antibody

• antigen recognized by monoclonal antibody 5.1H11 antibody

• CD56 antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [ERIC-1] (ab6123)

  Ab6123 staining Human normal skeletal muscle. Staining is localised the membrane.
  Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
  Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

• 

  Flow Cytometry - Anti-NCAM1 antibody [ERIC-1] (ab6123)

  Overlay histogram showing HCT116 cells stained with ab6123 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6123, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HCT116 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.