Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker

• 产品名称

  Anti-Desmin抗体[DE-U-10] - Cytoskeleton Marker
  参阅全部 Desmin 一抗

• 描述

  小鼠单克隆抗体[DE-U-10] to Desmin - Cytoskeleton Marker

• 宿主

  Mouse

• 经测试应用

  适用于: IHC-Pmore details
  不适用于: ICC/IF

• 种属反应性

  与反应: Mouse, Rat, Human
  预测可用于: Sheep, Rabbit, Goat, Chicken, Hamster, Cow, Cat, Lizard, Snake

• 免疫原

  Full length native protein (purified) corresponding to Pig Desmin.

• 阳性对照

• IHC-P: Human skeletal muscle, Mouse cardiac muscle and rat colon tissue.

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.02% Sodium azide
  Constituents: PBS, 6.97% L-Arginine

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Affinity purified

• 克隆

  单克隆

• 克隆编号

  DE-U-10

• 骨髓瘤

  unknown

• 同种型

  IgG1

• 轻链类型

  unknown

The Abpromise guarantee

Abpromise™承诺保证使用ab6322于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用说明

Is unsuitable for ICC/IF.

数据库链接

• Entrez Gene: 280765 Cow

• Entrez Gene: 1674 Human

• Entrez Gene: 13346 Mouse

• Entrez Gene: 64362 Rat

• Omim: 125660 Human

• SwissProt: P02542 Chicken

• SwissProt: O62654 Cow

• SwissProt: P17661 Human

• SwissProt: P31001 Mouse

• SwissProt: P48675 Rat

• Unigene: 594952 Human

• Unigene: 6712 Mouse

• Unigene: 39196 Rat

• 别名

  hide

• DES antibody

• DESM_HUMAN antibody

• Desmin antibody

• FLJ12025 antibody

• FLJ39719 antibody

• FLJ41013 antibody

• FLJ41793 antibody

• Intermediate filament protein antibody

• OTTHUMP00000064865 antibody

• CMD1I antibody

• CSM1 antibody

• CSM2 antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker (ab6322)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cardiac muscle tissue labelling Desmin with ab6322 at a dulution of 1/200. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). A ready to use HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody. Counter stained with Hematoxylin.

  Image shows cytomplasmic staining on mouse cardiac muscle.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker (ab6322)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat colon tissue labelling Desmin with ab6322 at a dulution of 1/200. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). A ready to use HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody. Counter stained with Hematoxylin.

  Image shows cytomplasmic staining on smooth muscle of rat colon.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker (ab6322)

  IHC image of Desmin staining in human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6322, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.