Anti-SERCA1 ATPase antibody [IIH11]

• 产品名称

  Anti-SERCA1 ATPase抗体[IIH11]
  参阅全部 SERCA1 ATPase 一抗

• 描述

  小鼠单克隆抗体[IIH11] to SERCA1 ATPase

• 宿主

  Mouse

• 经测试应用

  适用于: WB, IHC-Pmore details

• 种属反应性

  与反应: Mouse, Rat
  

• 免疫原

  Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

• 表位

  This antibody recognizes an epitope between amino acids 199-505 of rabbit skeletal muscle ATPase, a region that is not exposed in native sarcoplasmic reticulum.

• 阳性对照

• WB: Mouse and rat skeletal muscle tissue lysate. IHC-P: Mouse and rat skeletal muscle tissue.

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  This product is a recombinant monoclonal antibody, which offers several advantages including:

  For more information see here.

• - High batch-to-batch consistency and reproducibility

• - Improved sensitivity and specificity

• - Long-term security of supply

• - Animal-free production

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.2
  Preservative: 0.01% Sodium azide
  Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  IIH11

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab2818于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 11937 Mouse

• Entrez Gene: 116601 Rat

• SwissProt: Q8R429 Mouse

• SwissProt: Q64578 Rat

• Unigene: 35134 Mouse

• Unigene: 217139 Rat

• 别名

• ATP2A1 antibody

• ATPase Ca++ transporting cardiac muscle fast twitch 1 antibody

• ATPase Ca++ transporting fast twitch 1 antibody

• ATPase, Ca(2+)-transporting fast twitch 1 antibody

• Calcium pump 1 antibody

• Calcium transporting ATPase sarcoplasmic reticulum type fast twitch skeletal muscle isoform antibody

• Calcium-transporting ATPase sarcoplasmic reticulum type antibody

• EC 3.6.3.8 antibody

• Endoplasmic reticulum class 1/2 Ca(2+) ATPase antibody

• Fast skeletal muscle SR calcium ATPase antibody

• OTTHUMP00000162561 antibody

• OTTHUMP00000162562 antibody

• Sarcoendoplasmic reticulum calcium ATPase antibody

• Sarcoplasmic reticulum Ca(2+)-ATPase 1 antibody

• Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 antibody

• SERCA 1 antibody

• SERCA1 antibody

• SERCA1 truncated isoform, included antibody

• SR Ca(2+) ATPase 1 antibody

• SR Ca(2+)-ATPase 1 antibody

• fast twitch skeletal muscle isoform antibody

• AT2A1_HUMAN antibody

• ATP2A antibody

• 

  Western blot - Anti-SERCA1 ATPase antibody [IIH11] (ab2818)

  All lanes : Anti-SERCA1 ATPase antibody [IIH11] (ab2818) at 1/1000 dilution
  
  Lane 1 : Mouse skeletal muscle tissue lysate
  Lane 2 : Rat skeletal muscle tissue lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
  
  Predicted band size: 110 kDa
  Observed band size: 110 kDa
  
  
  Exposure time: 48 seconds
  
  
  

  Blocking/Dilution buffer: 5% NFDM/TBST.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [IIH11] (ab2818)

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling SERCA1 ATPase with ab2818 at 0.177µg/ml, followed by ready to use secondary. Cytoplasmic staining on rat skeletal muscle tissue is observed. The section was incubated with ab2818 for 15 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
  Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 mins.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [IIH11] (ab2818)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling SERCA1 ATPase with ab2818 at 0.117µg/ml, followed by ready to use secondary. Cytoplasmic staining on mouse skeletal muscle tissue is observed. The section was incubated with ab2818 for 15 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
  Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 mins.

• 

  Anti-SERCA1 ATPase antibody [IIH11] (ab2818)