Anti-alpha Adaptin antibody [AP6]

• 产品名称

  Anti-alpha Adaptin抗体[AP6]
  参阅全部 alpha Adaptin 一抗

• 描述

  小鼠单克隆抗体[AP6] to alpha Adaptin

• 宿主

  Mouse

• 特异性

  Detects assembly polypeptide 2 (AP2) It recognizes the products of both alpha-adaptin genes, alpha A and alpha C as well as an alternatively spliced isoform of alpha A found in neurons.

• 经测试应用

  适用于: ICC/IF, Flow Cytmore details

• 种属反应性

  与反应: Mouse, Human
  预测可用于: Non human primates

• 免疫原

  Full length native protein (purified) corresponding to alpha Adaptin. Purified adaptors.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.05% Sodium azide
  Constituent: PBS

• 浓度

• 100 µl 浓度为 1 mg/ml

• 纯度

  Affinity purified

• 克隆

  单克隆

• 克隆编号

  AP6

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab2730于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

  hide

• SwissProt: P17426 Mouse

• SwissProt: P17427 Mouse

• Entrez Gene: 160 Human

• Entrez Gene: 161 Human

• Entrez Gene: 11771 Mouse

• Entrez Gene: 11772 Mouse

• Omim: 601026 Human

• Omim: 607242 Human

• SwissProt: O94973 Human

• SwissProt: O95782 Human

别名

• 100 kDa coated vesicle protein A antibody

• 100 kDa coated vesicle protein C antibody

• Adapter related protein complex 2 subunit alpha 1 antibody

• Adapter related protein complex 2 subunit alpha 2 antibody

• Adaptin alpha A antibody

• Adaptin alpha B antibody

• Adaptin, alpha B antibody

• Adaptor protein complex AP 2 alpha 1 subunit antibody

• Adaptor protein complex AP 2 alpha 2 subunit antibody

• Adaptor protein complex AP 2 subunit alpha 1 antibody

• Adaptor related protein complex 2 alpha 1 subunit antibody

• Adaptor related protein complex 2 alpha 2 subunit antibody

• ADTAA antibody

• ADTAB antibody

• Alpha adaptin A antibody

• Alpha adaptin C antibody

• Alpha adaptin C; Huntingtin interacting protein J antibody

• Alpha1 adaptin antibody

• Alpha2 adaptin antibody

• AP 2 complex subunit alpha 1 antibody

• AP 2 complex subunit alpha 2 antibody

• AP2 alpha antibody

• AP2A1 antibody

• AP2A2 antibody

• CLAPA1 antibody

• CLAPA2 antibody

• Clathrin assembly protein complex 2 alpha A large chain antibody

• Clathrin assembly protein complex 2 alpha C large chain antibody

• Clathrin associated/assembly/adaptor protein large alpha 1 antibody

• Clathrin associated/assembly/adaptor protein large alpha 2 antibody

• HIP 9 antibody

• HIP9 antibody

• Huntingtin interacting protein antibody

• Huntingtin interacting protein HYPJ antibody

• Huntingtin interacting protein J antibody

• Huntingtin yeast partner J antibody

• HYPJ antibody

• Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit antibody

• Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (ab2730)

  Immunofluorescent analysis of alpha Adaptin using alpha Adaptin Monoclonal Antibody (AP6) (ab2730) shows staining in U251 Cells. alpha Adaptin (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing alpha Adaptin (ab2730) at a dilution of 1:20 over night at 4C and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (ab2730)

  Immunofluorescent analysis of alpha Adaptin using alpha Adaptin Monoclonal Antibody (AP6) (ab2720) shows staining in MCF-7 Cells. alpha Adaptin (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing alpha Adaptin (ab2730) at a dilution of 1:20 over night at 4 C and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (ab2730)

  Immunofluorescent analysis of alpha Adaptin using alpha Adaptin Monoclonal Antibody (AP6) (ab2730) shows staining in Hela Cells. alpha Adaptin (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing alpha Adaptin (ab2720) at a dilution of 1:20 over night at 4 C and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (ab2730)

  ICC/IF image of ab2730 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2730, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  
  

• 

  Flow Cytometry - Anti-alpha Adaptin antibody [AP6] (ab2730)

  Overlay histogram showing MCF7 cells stained with ab2730 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2730, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.