Anti-Nuclear Pore O-Linked Glycoprotein antibody [RL1]

• 产品名称

  Anti-Nuclear Pore O-Linked Glycoprotein抗体[RL1]

• 描述

  小鼠单克隆抗体[RL1] to核Pore O-Linked Glycoprotein

• 宿主

  Mouse

• 特异性

  Detects nuclear pore-O-linked glycoprotein

• 经测试应用

  适用于: IHC-P, WBmore details

• 种属反应性

  与反应: Rat, Human
  

• 免疫原

  Full length protein corresponding to Rat Nuclear Pore O-Linked Glycoprotein. Pore complex-lamina fraction purified from rat liver nuclear envelopes.

• 阳性对照

• WB: HEK-293, THP-1, HeLa and PC-12 cell lysates. IHC-P: Rat lymph node, kidney and brain tissue.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.05% Sodium azide
  Constituent: PBS

• 浓度

• 100 µl 浓度为 1 mg/ml

• 纯度

  Purified IgM

• 克隆

  单克隆

• 克隆编号

  RL1

• 同种型

  IgM

The Abpromise guarantee

Abpromise™承诺保证使用ab2734于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 

  Western blot - Anti-Nuclear Pore O-Linked Glycoprotein antibody [RL1] (ab2734)

  All lanes : Anti-Nuclear Pore O-Linked Glycoprotein antibody [RL1] (ab2734) at 1/1000 dilution
  
  Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
  Lane 2 : THP-1 (Human monocytic leukemia cell line) whole cell lysate
  Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
  Lane 4 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
  
  Lysates/proteins at 30 µg per lane.
  
  Secondary
  All lanes : Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution
  
  
  

  * an uncharacterized band at ~45 kDa. 

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Pore O-Linked Glycoprotein antibody [RL1] (ab2734)

  Immunohistochemistry was performed on normal biopsies of deparaffinized Rat lymph node tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Nuclear Pore-O-Linked Glycoprotein ab2734 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Pore O-Linked Glycoprotein antibody [RL1] (ab2734)

  Immunohistochemistry was performed on normal biopsies of deparaffinized Rat brain tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Nuclear Pore-O-Linked Glycoprotein ab2734 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Pore O-Linked Glycoprotein antibody [RL1] (ab2734)

  Immunohistochemistry was performed on normal biopsies of deparaffinized Rat kidney tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Nuclear Pore-O-Linked Glycoprotein ab2734 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.