Anti-CD14抗体[MEM-18] - Low endotoxin，Azide free
参阅全部 CD14 一抗
小鼠单克隆抗体[MEM-18] to CD14 - Low endotoxin，Azide free
Mouse
适用于: Flow Cytmore details
与反应: Human
预测可用于: Monkey, Non human primates
Full length protein corresponding to Human CD14. A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.40
Constituent: PBS
是
Tissue culture supernatant
单克隆
MEM-18
IgG1
Abpromise™承诺保证使用ab6083于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt | Use 0.01µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Entrez Gene: 929 Human
Omim: 158120 Human
SwissProt: P08571 Human
Unigene: 163867 Human
CD14 antigen antibody
CD14 molecule antibody
CD14_HUMAN antibody
LPS-R antibody
Mo2 antibody
Monocyte differentiation antigen CD14 antibody
Monocyte differentiation antigen CD14 urinary form antibody
Monocyte differentiation antigen CD14, membrane-bound form antibody
Myeloid cell specific leucine rich glycoprotein antibody
Myeloid cell-specific leucine-rich glycoprotein antibody
CD 14 antibody
CD_antigen=CD14 antibody
CD14 antibody
![Flow Cytometry - Anti-CD14 antibody [MEM-18] - Low endotoxin, Azide free (ab6083)](https://www.abcam.cn/ps/products/6/ab6083/Images/ab6083-199461-anti-cd14-antibody-mem-18-flow-cytometry.jpg)
Flow Cytometry - Anti-CD14 antibody [MEM-18] - Low endotoxin, Azide free (ab6083)
Human peripheral blood cells stained with ab6083 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab6083, 0.01μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
