Anti-SERCA1 ATPase antibody [VE121G9]

• 产品名称

  Anti-SERCA1 ATPase抗体[VE121G9]
  参阅全部 SERCA1 ATPase 一抗

• 描述

  小鼠单克隆抗体[VE121G9] to SERCA1 ATPase

• 宿主

  Mouse

• 特异性

  Detects Sarcoplasmic or Endoplasmic Reticulum Calcium 1 (SERCA 1) ATPase.

• 经测试应用

  适用于: IHC-Fr, WB, IHC-Pmore details

• 种属反应性

  与反应: Mouse, Human
  

• 免疫原

  Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.

• 表位

  This antibody recognizes an epitope between amino acid residues 506 and the C-terminus of rabbit skeletal muscle ATPase, a region that is exposed in native sarcoplasmic reticulum.

• 阳性对照

• WB: Normal mouse and human skeletal muscle IHC-P: Normal mouse and human skeletal muscle IHC-Fr: Normal mouse and human skeletal muscle

• 常规说明

   This product was switched from a hybridoma to a recombinant production format on 25th October 2021.

   

  This product is a recombinant monoclonal antibody, which offers several advantages including:

  For more information see here.

• - High batch-to-batch consistency and reproducibility

• - Improved sensitivity and specificity

• - Long-term security of supply

• - Animal-free production

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.20
  Preservative: 0.01% Sodium azide
  Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

• 浓度

• 10 µg 浓度为 1.04 mg/ml

• 100 µg 浓度为 1.04 mg/ml

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  VE121G9

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab2819于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 487 Human

• Entrez Gene: 11937 Mouse

• Omim: 108730 Human

• SwissProt: O14983 Human

• SwissProt: Q8R429 Mouse

• Unigene: 657344 Human

• Unigene: 35134 Mouse

• 别名

• ATP2A1 antibody

• ATPase Ca++ transporting cardiac muscle fast twitch 1 antibody

• ATPase Ca++ transporting fast twitch 1 antibody

• ATPase, Ca(2+)-transporting fast twitch 1 antibody

• Calcium pump 1 antibody

• Calcium transporting ATPase sarcoplasmic reticulum type fast twitch skeletal muscle isoform antibody

• Calcium-transporting ATPase sarcoplasmic reticulum type antibody

• EC 3.6.3.8 antibody

• Endoplasmic reticulum class 1/2 Ca(2+) ATPase antibody

• Fast skeletal muscle SR calcium ATPase antibody

• OTTHUMP00000162561 antibody

• OTTHUMP00000162562 antibody

• Sarcoendoplasmic reticulum calcium ATPase antibody

• Sarcoplasmic reticulum Ca(2+)-ATPase 1 antibody

• Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 antibody

• SERCA 1 antibody

• SERCA1 antibody

• SERCA1 truncated isoform, included antibody

• SR Ca(2+) ATPase 1 antibody

• SR Ca(2+)-ATPase 1 antibody

• fast twitch skeletal muscle isoform antibody

• AT2A1_HUMAN antibody

• ATP2A antibody

• 

  Western blot - Anti-SERCA1 ATPase antibody [VE121G9] (ab2819)

  All lanes : Anti-SERCA1 ATPase antibody [VE121G9] (ab2819) at 1 µg/ml
  
  Lane 1 : Human Skeletal Muscle tissue lysate
  Lane 2 : Mouse Skeletal Muscle tissue lysate
  Lane 3 : Human Brain tissue lysate
  Lane 4 : Mouse Brain tissue lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  All lanes : Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) at 1/20000 dilution
  
  Predicted band size: 110 kDa
  Observed band size: 110 kDa
  
  
  
  

  This blot was produced using 3-8% Tris-Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was blocked for an hour using 3% milk before ab2819 was incubated overnight at 4°C at a 1µg/ml concentration. Antibody binding was detected using Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibody at 1/20000 dilution for 1 hour at room temperature before imaging.

• 

  Immunohistochemistry (Frozen sections) - Anti-SERCA1 ATPase antibody [VE121G9] (ab2819)

  IHC image of SERCA1 ATPase staining in a section of frozen normal mouse skeletal muscle performed on a Leica BOND™ system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab2819, 1µg/ml for 15 mins at room temperature and then ab125913, Goat anti-Mouse IgG1 at 1.5ugml was added for 15 mins at room temperature. This was detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• 

  Immunohistochemistry (Frozen sections) - Anti-SERCA1 ATPase antibody [VE121G9] (ab2819)

  IHC image of SERCA1 ATPase staining in a section of frozen normal human skeletal muscle* performed on a Leica BOND™ system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab2819, 1µg/ml for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [VE121G9] (ab2819)

  IHC image of SERCA1 ATPase staining in a section of formalin-fixed paraffin-embedded normal human skeletal muscle* performed on a Leica BOND™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab2819, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [VE121G9] (ab2819)

  IHC image of SERCA1 ATPase staining in a section of formalin-fixed paraffin-embedded normal mouse skeletal muscle performed on a Leica BOND™ system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab2819, 1ug/ml, for 15 mins at room temperature and then ab125913, Goat anti-Mouse IgG1 at 1.5ugml was added for 15 mins at room temperature. This was detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• 

  Western blot - Anti-SERCA1 ATPase antibody [VE121G9] (ab2819)This image is courtesy of an anonymous Abreview

  Anti-SERCA1 ATPase antibody [VE121G9] (ab2819) at 1/1000 dilution (in PBS tweeb 0.05% for 1 hour at 22°C) + Whole tissue lysate of human neck muscle. at 20 µg
  
  Secondary
  An HRP-conjugated sheep anti-mouse polyclonal at 1/4000 dilution
  
  Developed using the ECL technique.
  
  Predicted band size: 110 kDa
  Observed band size: 110 kDa
  
  
  Exposure time: 5 minutes
  
  
  

  Blocking Step: 5% milk for 16 hours at 22°C

   

  This image was generated from the Hybridoma version. 

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [VE121G9] (ab2819)

  Negative control image: IHC image of SERCA1 ATPase staining in a section of formalin-fixed paraffin-embedded normal mouse cerebellum performed on a Leica BOND™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2819, 1ug/ml, for 15 mins at room temperature and then ab125913, Goat anti-Mouse IgG1 at 1.5ugml was added for 15 mins at room temperature. This was detected using an HRP conjugated compact polymer system DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [VE121G9] (ab2819)

  Negative control image: IHC image of SERCA1 ATPase staining in a section of formalin-fixed paraffin-embedded normal human cerebellum* performed on a Leica BOND™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab2819, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• 

  Immunohistochemistry (Frozen sections) - Anti-SERCA1 ATPase antibody [VE121G9] (ab2819)

  Negative control image: IHC image of SERCA1 ATPase staining in a section of frozen normal human cerebral cortex* performed on a Leica BOND™ system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab2819, 1ug/ml for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• 

  Immunohistochemistry (Frozen sections) - Anti-SERCA1 ATPase antibody [VE121G9] (ab2819)

  Negative control image: IHC image of SERCA1 ATPase staining in a section of frozen normal mouse cerebral cortex performed on a Leica BOND™ system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab2819, 1ug/ml for 15 mins at room temperature and then ab125913, Goat anti-Mouse IgG1 at 1.5ugml was added for 15 mins at room temperature. This was detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.