Anti-Cathepsin D抗体[CTD-19]
参阅全部 Cathepsin D 一抗
小鼠单克隆抗体[CTD-19] to Cathepsin D
Mouse
适用于: WBmore details
与反应: Human
Full length native protein (purified) corresponding to Human Cathepsin D.
Database link: P07339
Human breast carcinoma tissue or cell line extract.
This product was changed from ascites to tissue culture supernatant on 21 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.097% Sodium azide
浓度
批次浓度范围 50 µl 浓度为 1.1 - 1.4 mg/ml
Proprietary Purification
Purified from TCS
单克隆
CTD-19
IgG2a
Abpromise™承诺保证使用ab6313于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (12) | Use at an assay dependent concentration. Detects a band of approximately 52 kDa. |
Entrez Gene: 1509 Human
Omim: 116840 Human
SwissProt: P07339 Human
Unigene: 654447 Human
Cathepsin D heavy chain antibody
CD antibody
Ceroid lipofuscinosis neuronal 10 antibody
CLN10 antibody
CPSD antibody
ctsd antibody
Epididymis secretory sperm binding protein Li 130P antibody
HEL S 130P antibody
Lysosomal aspartyl peptidase antibody
Lysosomal aspartyl protease antibody
MGC2311 antibody
CatD antibody
CATD_HUMAN antibody
Cathepsin D antibody
Western blot - Anti-Cathepsin D antibody [CTD-19] (ab6313)This image is courtesy of an anonymous Abreview
Anti-Cathepsin D antibody [CTD-19] (ab6313) at 1/1000 dilution + MDA MB 231 whole cell lysate at 20 µg
Secondary
HRP-conjugated monoclonal sheep anti-mouse IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 28,48,50 kDawhy is the actual band size different from the predicted?
Exposure time: 2 minutes
Blocked with 5% milk for 1 hour at 22°C.
Incubated with the primary antibody for 1 hour at 22°C in 5% TBS-T.
This image was generated using the ascites version of the product.
Western blot - Anti-Cathepsin D antibody [CTD-19] (ab6313)
Anti-Cathepsin D antibody [CTD-19] (ab6313) at 1/4000 dilution + MCF7 Human breast carcinoma cells
This image was generated using the ascites version of the product.