Anti-CEACAM5 antibody [26/3/13] - BSA and Azide free

• 产品名称

  Anti-CEACAM5抗体[26/3/13] - BSA and Azide free
  参阅全部 CEACAM5 一抗

• 描述

  小鼠单克隆抗体[26/3/13] to CEACAM5 - BSA and Azide free

• 宿主

  Mouse

• 特异性

  This antibody recognises exclusively CEACAM 5 transiently expressed on the cell surface of transfected BOSC cells. It can be used to distinguish CEACAM 5 from all other CEACAM and probably all pregnancy-specific glycoproteins molecules, namely CEACAM 1 (BGP/CD66a), CEACAM 3 (CGM1/CD66d), CEACAM 4 (CGM7), CEACAM 6 (NCA/CD66c), CEACAM 7 (CGM2), CEACAM 8 (CGM6/CD66b)and PSG1 (CD66f) based on its reactivity pattern with stable HeLa transfectants expressing individual CEA family members. This antibody was included and characterized in the studies from the VIth Leucocyte Typing Workshop (Grunert F et al. 1994)

• 经测试应用

  适用于: Indirect ELISA, IHC-Pmore details
  不适用于: Flow Cyt or WB

• 种属反应性

  与反应: Human
  不与反应: Mouse, Rat

• 免疫原

  Full length native protein (partially purified) (Human) from a perchloric acid extract from liver metastases of colonic tumors (Grunert F, et al. 1985)

• 常规说明

  Antibodies produced from cDNA: Conventional technologies usually either generate antibodies against purified proteins, or against synthetic peptides based on amino acid sequences derived from DNA sequence data. Genetic immunization involves introducing the gene in the form of a cDNA directly into an animal which translates this cDNA into protein thus stimulating an immune response against the foreign protein. Although the synthetic peptide approach is comparable in speed, the quality of antibodies generated by genetic immunization is far superior. This is because the protein is made by the immunized animal, utilzing complex cellular mechanisms that allow it to gain a native conformation. Antibodies are then generated against a native protein, such as is found in the blood or tissues of its host species. Membrane-bound or secreted proteins often create problems for conventional antibody technology because in their native form, they are often modified by glycosylation, or in some cases exist as multiple membrane-spanning proteins that are not soluble following isolation or synthesis in recombinant systems. All of these problems are avoided if the immunized animal makes the protein itself. Antibodies generated by genetic immunization have been shown to have binding affinities to the protein in the sub-nanomolar range, which are approximately 100x higher than conventionally developed antibodies and much higher than single chain antibodies. Results confirm published data for much higher avidity of sera generated by genetic immunization as compared with that gained by immunization with a corresponding recombinant protein.

  This product has switched from a hybridoma to recombinant production method on 20th June 2025

  This product is a recombinant monoclonal antibody, which offers several advantages including:

  For more information see here.

• - High batch-to-batch consistency and reproducibility

• - Improved sensitivity and specificity

• - Long-term security of supply

• - Animal-free production

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.20
  Constituent: 100% PBS

• 无载体

  是

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  26/3/13

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab4451于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用说明

Is unsuitable for Flow Cyt or WB.

• 数据库链接

• Entrez Gene: 1048 Human

• Omim: 114890 Human

• SwissProt: P06731 Human

• Unigene: 709196 Human

• 别名

• CEA antibody

• Ceacam5 antibody

• CEAM5_HUMAN antibody

• DKFZp781M2392 antibody

• Meconium antigen 100 antibody

• OTTHUMP00000199032 antibody

• OTTHUMP00000199033 antibody

• OTTHUMP00000199034 antibody

• Carcinoembryonic antigen antibody

• Carcinoembryonic antigen-related cell adhesion molecule 5 antibody

• CD66e antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 antibody [26/3/13] - BSA and Azide free (ab4451)

  IHC image of Carcino Embryonic Antigen CEA staining in human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4451, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• 

  Sandwich ELISA - Anti-CEACAM5 antibody [26/3/13] - BSA and Azide free (ab4451)

  Standard Curve for Carcino Embryonic Antigen CEA dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [26/3/13] to Carcino Embryonic Antigen CEA (ab4451) at 0.2ug/ml and Detector Antibody Rabbit polyclonal to Carcino Embryonic Antigen CEA (ab15987) at 0.5ug/ml.