Anti-Leptin抗体
参阅全部 Leptin 一抗
兔多克隆抗体to Leptin
Rabbit
适用于: ICC, IHC-Fr, Flow Cytmore details
与反应: Mouse, Sheep, Human
预测可用于: Goat, Horse, Chicken, Cow, Cat, Dog, Turkey, Chimpanzee, Rhesus monkey
Synthetic peptide corresponding to Mouse Leptin aa 25-44.
Sequence:
QKVQDDTKTLIKTIVTRIND
ICC: 3T3-L1, HeLa and mesenchymal stem cells. IHC-P: Sheep brain tissue. Flow Cyt: 3T3-L1 cells.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
浓度
50 µl 浓度为 2 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab3583于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC | Use a concentration of 5 µg/ml. | |
| IHC-Fr | (1) | 1/50 - 1/200. |
| Flow Cyt | Use 3-5µl for 106 cells. |
Entrez Gene: 373955 Chicken
Entrez Gene: 449638 Chimpanzee
Entrez Gene: 3952 Human
Entrez Gene: 16846 Mouse
Entrez Gene: 698728 Rhesus monkey
Omim: 164160 Human
SwissProt: O42164 Chicken
SwissProt: O02750 Chimpanzee
SwissProt: Q257X2 Goat
SwissProt: Q9TU09 Horse
SwissProt: P41159 Human
SwissProt: P41160 Mouse
SwissProt: Q28504 Rhesus monkey
SwissProt: Q28603 Sheep
Unigene: 194236 Human
Unigene: 277072 Mouse
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Immunocytochemistry - Anti-Leptin antibody (ab3583)
ab3583 staining Leptin in 3T3-L1 cells by ICC (Immunocytochemistry). Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (2ug/ml in 0.1% BSA) for 3 hours at room temperature. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG (H+L) polyclonal was used as the secondary antibody (1/2000) (Panel a). Nuclei were stained with DAPI (Panel b). F-actin stained with Rhodamine Phalloidin (panel c). Merged images shown in panel d.
Immunohistochemistry (Frozen sections) - Anti-Leptin antibody (ab3583)
Immunohistochemical staining of Leptin in Sheep brain using ab3583.
Immunocytochemistry - Anti-Leptin antibody (ab3583)
ICC image of ab3583 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3583, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunocytochemistry - Anti-Leptin antibody (ab3583)This image is courtesy of an Abreview submitted by Steven Johnstone
ab3583 staining Leptin in Human mesenchyml stem cells by ICC (Immunocytochemistry). Cells were fixed with paraformaldehyde and blocked with 10% serum for 30 minutes at 22°C. Samples were incubated with primary antibody (1/100 in 10% horse serum) for 1 hour at 22°C. A FITC-conjugated Mouse anti-rabbit IgG1 monoclonal was used as the secondary antibody (1/100).
Flow Cytometry - Anti-Leptin antibody (ab3583)
ab3583 staining Leptin in 3T3-L1 cells by Flow Cytometry. The sample was incubated with the primary antibody (3ug/ml in 2.5% BSA) for 2 hours at room temperature. An Alexa Fluor 488®-conjugated Goat anti-rabbit (1/400) was used as the secondary antibody. Red histogram represents ab3583, pink histogram represents isotype control, purple histogram represents unstained control, green histogram represents no primary antibody control
