Anti-KIF4A/KIF4抗体
参阅全部 KIF4A/KIF4 一抗
山羊多克隆抗体to KIF4A/KIF4
Goat
适用于: ICC/IF, WBmore details
与反应: Mouse, Human
预测可用于: Rat, Cow, Pig
Synthetic peptide corresponding to Human KIF4A/KIF4 aa 1219-1232 (C terminal).
Sequence:
SFFSGCSPIEEEAH
(Peptide available as ab22955)
WB: NIH/3T3 nuclear lysate. ICC/IF: U-2 OS cells.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.30
Preservative: 0.02% Sodium azide
Constituent: 0.5% BSA
浓度
100 µg 浓度为 0.5 mg/ml
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
多克隆
IgG
Abpromise™承诺保证使用ab3815于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (2) | Use a concentration of 10 µg/ml. |
| WB | (1) | Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 140 kDa. |
Entrez Gene: 24137 Human
Entrez Gene: 16571 Mouse
NCBI: 7305205 Human
Omim: 300521 Human
SwissProt: O95239 Human
SwissProt: P33174 Mouse
Unigene: 648326 Human
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Western blot - Anti-KIF4A/KIF4 antibody (ab3815)
All lanes : Anti-KIF4A/KIF4 antibody (ab3815) at 2 µg/ml
Lane 1 : NIH/3T3 nuclear lysate
Lane 2 : Human ovary lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 140 kDa
Immunocytochemistry/ Immunofluorescence - Anti-KIF4A/KIF4 antibody (ab3815)
Immunocytochemical analysis of paraformaldehyde-fixed U-2 OS cells permeabilized with 0.15% Triton. Cells were incubated with ab3815 at 10 ug/ml for 1 hour followed by an Alexa Fluor® 488 secondary at 4 ug/ml (green). The nuclear stain is DAPI (blue). Negative control is unimmunized goat IgG at 10 ug/ml, followed by an Alexa Fluor® 488 secondary at 4 ug/ml (green).
