山羊多克隆抗体to AP-2 complex subunit alpha-1
Goat
The immunogen sequence is found in both AP2A2 and AP2A1. This antibody recognises a band of just under 100kD in multiple human cell lines (see picture), the band can be blocked with the immunising peptide.
适用于: WBmore details
与反应: Human
预测可用于: Mouse
Synthetic peptide corresponding to Human AP-2 complex subunit alpha-1 aa 3-14.
Sequence:
AVSKGDGMRGLAC
(Peptide available as ab11698)
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride
浓度
100 µg 浓度为 1.7 mg/ml
Immunogen affinity purified
This affinity purified antibody is directed against human AP2A. The product was affinity purified from antiserum by immunoaffinity purification.
多克隆
IgG
Abpromise™承诺保证使用ab3707于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/500 - 1/2000. Detects a band of approximately 100 kDa (predicted molecular weight: 104 , 108 kDa). |
Entrez Gene: 160 Human
Entrez Gene: 11771 Mouse
Omim: 601026 Human
SwissProt: O95782 Human
SwissProt: P17426 Mouse
Unigene: 467125 Human
Unigene: 6877 Mouse
Adaptin alpha A antibody
Adaptin alpha antibody
Adaptor protein complex AP 2 alpha 1 subunit antibody
adaptor protein complex AP 2 subunit alpha 1 antibody
Adaptor protein complex AP-2 subunit alpha-1 antibody
Adaptor protein complex AP2 alpha 1 subunit antibody
adaptor protein complex AP2 subunit alpha 1 antibody
Adaptor related protein complex 2 alpha 1 subunit antibody
Adaptor related protein complex AP 2 alpha 1 subunit antibody
Adaptor related protein complex AP2 alpha 1 subunit antibody
ADTAA antibody
Alpha A adaptin antibody
Alpha adaptin A antibody
Alpha-adaptin A antibody
alpha1 adaptin antibody
Alpha1-adaptin antibody
AP 2 alpha antibody
AP 2 complex subunit alpha 1 antibody
AP-2 complex subunit alpha-1 antibody
AP2 complex subunit alpha 1 antibody
AP2-ALPHA antibody
Ap2a1 antibody
AP2A1_HUMAN antibody
CLAPA 1 antibody
CLAPA1 antibody
Clathrin adaptor complex AP2. alpha subunit antibody
Clathrin assembly protein complex 2 alpha A large chain antibody
Clathrin assembly protein complex 2 alpha-A large chain antibody
Clathrin associated / assembly / adaptor protein large alpha 1 antibody
clathrin associated assembly adaptor protein large alpha 1 antibody
clathrin associated/assembly/adaptor protein, large, alpha 1 antibody
Plasma membrane adaptor HA 2/AP 2 adaptin alpha A subunit antibody
Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit antibody
100 kDa coated vesicle protein A antibody
Adapter-related protein complex 2 alpha-1 subunit antibody
adapter-related protein complex 2 subunit alpha-1 antibody
Western blot - Anti-AP-2 complex subunit alpha-1 antibody (ab3707)
Western blot using ab3707 at 1/500.
Lane 1: HeLa Nuclear Extract
Lane 2: HeLa Whole Cell Lysate
Lane 3: 293 Whole Cell Lysate
Lane 4: A431 Whole Cell Lysate
Lane 5: Jurkat Whole Cell Lysate
Lane 6: HeLa Nuclear Extract + blocking/immunising peptide
Lane 7: HeLa Whole Cell Lysate + blocking/immunising peptide
Lane 8: 293 Whole Cell Lysate + blocking/immunising peptide
Lane 9: A431 Whole Cell Lysate + blocking/immunising peptide
Lane 10: Jurkat Whole Cell Lysate + blocking/immunising peptide
AP2 alpha proteins (AP2A2 and AP2A1) have predicted molecular weights of 104 and 107kD. The band at just below 97kD (that is blocked by the immunising peptide) represents AP2 alpha, the lower bands are not blocked completely by the immunising peptide and are believed to be non-specific.
Secondary ab: Rabbit polyclonal to Goat IgG HRP
ab6741 (1/5000)
Exposure time: 1 minute.<
