Anti-SNF2H抗体- ChIP Grade
参阅全部 SNF2H 一抗
兔多克隆抗体to SNF2H - ChIP Grade
Rabbit
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab183730.
适用于: IP, ICC/IF, ChIP, WBmore details
与反应: Mouse, Human
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
浓度
批次浓度范围 100 µg 浓度为 0.8 - 0.9 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab3749于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | (1) | Use at an assay dependent concentration. PubMed: 16917504 |
| ICC/IF | (3) | Use at an assay dependent concentration. |
| ChIP | (1) | Use 2.5µl for 106 cells. |
| WB | (4) | 1/500. Detects a band of approximately 120 kDa (predicted molecular weight: 122 kDa). |
Entrez Gene: 8467 Human
Entrez Gene: 93762 Mouse
Omim: 603375 Human
SwissProt: O60264 Human
SwissProt: Q91ZW3 Mouse
Unigene: 558422 Human
Unigene: 246803 Mouse
Unigene: 474173 Mouse
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Western blot - Anti-SNF2H antibody - ChIP Grade (ab3749)
Rabbit polyclonal ab3749 to SNF2H was used in all lanes at 1/500 dilution.
Lane 1: HeLa lysate (20ug)
Lane 2: Mouse 3T3 lysate (20ug)
Lane 3: HeLa lysate (20ug), blocking peptide (ab5160) 1.0ug
Lane 4: Mouse 3T3 lysate (20ug), blocking peptide (ab5160) 1.0ug
Secondary antibody: Goat polyclonal to Rabbit IgG (ab6721)
ab3749 was able to recognise SNF2H in HeLA and Mouse 3T3 lysates (band at ~120 kDa). Binding was blocked by the immunising peptide ab5160.
Rabbit polyclonal ab3749 to SNF2H was used in all lanes at 1/500 dilution.
Lane 1: HeLa lysate (20ug)
Lane 2: Mouse 3T3 lysate (20ug)
Lane 3: HeLa lysate (20ug), blocking peptide (ab5160) 1.0ug
Lane 4: Mouse 3T3 lysate (20ug), blocking peptide (ab5160) 1.0ug
Secondary antibody: Goat polyclonal to Rabbit IgG (ab6721)
ab3749 was able to recognise SNF2H in HeLA and Mouse 3T3 lysates (band at ~120 kDa). Binding was blocked by the immun
ChIP - Anti-SNF2H antibody - ChIP Grade (ab3749)
ab3749 works in ChIP, as shown by the detection of an increase in the recruitment of SNF2H to the estrogen-responsive pS2 promoter.
Sonicated Chromatin prepared from untreated or 17beta-estradiol (E) treated MCF7 cells was subjected to the ChIP procedure with ab3749 antibody specific to SNF2H. The immunoprecipitated chromatin was analysed in the proximal region of the estro
