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Anti-KIF14 antibody

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  • 产品名称

    Anti-KIF14抗体
    参阅全部 KIF14 一抗

  • 描述

    兔多克隆抗体to KIF14

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WBIPmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide corresponding to Human KIF14 aa 900-1000.
    Database link: 
    Q15058

  • 阳性对照

    • WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.

  • 常规说明


    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • 存储溶液

    pH: 7
    Preservative: 0.1% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris

  • 纯度

    Immunogen affinity purified

  • 克隆

    多克隆

  • 同种型

    IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab3747于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用Ab评论说明
WB

1/2000 - 1/10000. Predicted molecular weight: 190 kDa.

IP

Use at 1-4 µg/mg of lysate.

  • 数据库链接

  • 别名

    • KIF14_HUMAN antibody

    • Kinesin Family Member 14 antibody

    • Kinesin like protein KIF14 antibody

    • Kinesin-like protein KIF14 antibody

    • MGC142302 antibody

    • KIAA0042 antibody

    • KIF 14 antibody

    • KIF14 antibody

  • Western blot - Anti-KIF14 antibody (ab3747)

    Western blot - Anti-KIF14 antibody (ab3747)

    All lanes : Anti-KIF14 antibody (ab3747) at 1/2000 dilution

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Predicted band size: 190 kDa


    Exposure time: 10 seconds

  • Immunoprecipitation - Anti-KIF14 antibody (ab3747)

    Immunoprecipitation - Anti-KIF14 antibody (ab3747)

    Whole cell lysate (50 µg for input; 1 mg/IP, 20% of IP loaded) from HeLa cells. ab3747 used for western blot at 1 µg/ml and used for IP at 3 µg/mg lysate. Detection: Chemiluminescence with an exposure time of 30 seconds.

    Lane 1 - Input.

    Lane 2 - IP.

    Lane 3 - Control IgG IP.