Anti-KIF14抗体
参阅全部 KIF14 一抗
兔多克隆抗体to KIF14
Rabbit
适用于: WB, IPmore details
与反应: Human
Synthetic peptide corresponding to Human KIF14 aa 900-1000.
Database link: Q15058
WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab3747于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | 1/2000 - 1/10000. Predicted molecular weight: 190 kDa. | |
IP | Use at 1-4 µg/mg of lysate. |
Entrez Gene: 9928 Human
Omim: 611279 Human
SwissProt: Q15058 Human
Unigene: 3104 Human
KIF14_HUMAN antibody
Kinesin Family Member 14 antibody
Kinesin like protein KIF14 antibody
Kinesin-like protein KIF14 antibody
MGC142302 antibody
KIAA0042 antibody
KIF 14 antibody
KIF14 antibody
Western blot - Anti-KIF14 antibody (ab3747)
All lanes : Anti-KIF14 antibody (ab3747) at 1/2000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 190 kDa
Exposure time: 10 seconds
Immunoprecipitation - Anti-KIF14 antibody (ab3747)
Whole cell lysate (50 µg for input; 1 mg/IP, 20% of IP loaded) from HeLa cells. ab3747 used for western blot at 1 µg/ml and used for IP at 3 µg/mg lysate. Detection: Chemiluminescence with an exposure time of 30 seconds.
Lane 1 - Input.
Lane 2 - IP.
Lane 3 - Control IgG IP.