Anti-pan CEACAM antibody [D14HD11]

• 产品名称

  Anti-pan CEACAM抗体[D14HD11]
  参阅全部 pan CEACAM 一抗

• 描述

  小鼠单克隆抗体[D14HD11] to pan CEACAM

• 宿主

  Mouse

• 特异性

  Broadly reactive with high affinity (affinity to solid phase bound CEACAM 5: 2 x 1010 L/Mol [Jantscheff P et al.]) with most of the CEACAM molecules (except CEACAM 7 and CEACAM 8) transiently expressed on the cell surface of transfected BOSC23 cells. This antibody can be used to detect CEACAM 1 (BGP/CD66a), CEACAM 3 (CGM1/CD66d), CEACAM 4 (CGM 7), CEACAM 5 and CEACAM 6 (NCA/CD66c) on transfectants by flow cytometry.

• 经测试应用

  适用于: ELISA, IHC-Fr, IHC-P, Flow Cyt, WBmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Full length native Carcino Embryonic Antigen (partially purified) (Human) from a perchloric acid extract from liver metastases of colonic tumors (Jantscheff P et al.).

• 常规说明

  Antibodies produced from cDNA: Conventional technologies usually either generate antibodies against purified proteins, or against synthetic peptides based on amino acid sequences derived from DNA sequence data. Genetic immunization involves introducing the gene in the form of a cDNA directly into an animal which translates this cDNA into protein thus stimulating an immune response against the foreign protein. Although the synthetic peptide approach is comparable in speed, the quality of antibodies generated by genetic immunization is far superior. This is because the protein is made by the immunized animal, utilzing complex cellular mechanisms that allow it to gain a native conformation. Antibodies are then generated against a native protein, such as is found in the blood or tissues of its host species. Membrane-bound or secreted proteins often create problems for conventional antibody technology because in their native form, they are often modified by glycosylation, or in some cases exist as multiple membrane-spanning proteins that are not soluble following isolation or synthesis in recombinant systems. All of these problems are avoided if the immunized animal makes the protein itself. Antibodies generated by genetic immunization have been shown to have binding affinities to the protein in the sub-nanomolar range, which are approximately 100x higher than conventionally developed antibodies and much higher than single chain antibodies. Results confirm published data for much higher avidity of sera generated by genetic immunization as compared with that gained by immunization with a corresponding recombinant protein.

  
  

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• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.01% Sodium azide
  Constituent: PBS

• 浓度

• 100 µg 浓度为 2 mg/ml

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  D14HD11

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab4567于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 1048 Human

• Entrez Gene: 1084 Human

• Entrez Gene: 1089 Human

• Entrez Gene: 4680 Human

• Entrez Gene: 634 Human

• SwissProt: O75871 Human

• SwissProt: P06731 Human

• SwissProt: P40198 Human

• SwissProt: P40199 Human

别名

• Antigen CD66 antibody

• Biliary glycoprotein antibody

• Carcinoembryonic antigen related cell adhesion molecule 1 antibody