Anti-Histone H1.0 antibody [27]

• 产品名称

  Anti-Histone H1.0抗体[27]
  参阅全部 Histone H1.0 一抗

• 描述

  小鼠单克隆抗体[27] to Histone H1.0

• 宿主

  Mouse

• 经测试应用

  适用于: WB, IHC-Pmore details
  不适用于: Flow Cyt (Intra)

• 种属反应性

  与反应: Human
  预测可用于: Mouse, Rat, Cow, Xenopus laevis, Vertebrata不与反应: Bird

• 免疫原

  Full length native protein (purified) corresponding to Cow Histone H1.0.

• 表位

  This antibody recognises an epitope within aa24-30. Proline 26, which is responsible for a bend in this region, plays an important role in the recognition. See Gorka et al. 1998 for more information.

• 阳性对照

• WB: A431, MCF7 and HeLa cell lysates; Histone H1.0 Human Recombinant Protein. IHC-P: Human colon and pancreas adenocarcinoma tissues.

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.02% Sodium azide
  Constituents: PBS, 6.97% L-Arginine

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Affinity purified

• 克隆

  单克隆

• 克隆编号

  27

• 骨髓瘤

  NS1/1-Ag4-1

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab11080于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用说明

Is unsuitable for Flow Cyt (Intra).

数据库链接

• Entrez Gene: 3005 Human

• Entrez Gene: 14958 Mouse

• Entrez Gene: 24437 Rat

• Omim: 142708 Human

• SwissProt: Q0IIJ2 Cow

• SwissProt: P07305 Human

• SwissProt: P10922 Mouse

• SwissProt: P43278 Rat

• Unigene: 745024 Human

• Unigene: 24350 Mouse

• Unigene: 3129 Rat

别名

• H1 histone family member 0 antibody

• H1(0) antibody

• H10 antibody

• H10_HUMAN antibody

• h1f0 antibody

• H1FV antibody

• Histone H1'' antibody

• Histone H1(0) antibody

• Histone H1.0 antibody

• Histone H10 antibody

• Histone H5 antibody

• MGC5241 antibody

• N-terminally processed antibody

• 

  Western blot - Anti-Histone H1.0 antibody [27] (ab11080)

  All lanes : Anti-Histone H1.0 antibody [27] (ab11080) at 1/500 dilution
  
  Lane 1 : Wild-type A431 cell lysate
  Lane 2 : H1F0 knockout A431 cell lysate
  Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
  
  Lysates/proteins at 40 µg per lane.
  
  Performed under reducing conditions.
  
  Predicted band size: 20 kDa
  Observed band size: 30 kDawhy is the actual band size different from the predicted?
  
  
  
  

  Lanes 1 - 3: Merged signal (red and green). Green - ab11080 observed at 30 kDa. Red - loading control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55 kDa.

  ab11080 was shown to react with Histone H1.0 in wild-type A431 cells in Western blot with loss of signal observed in H1F0 knockout sample. Wild-type A431 and H1F0 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab11080 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody [27] (ab11080)

  IHC image of Histone H1 staining in a section of formalin-fixed paraffin-embedded [human normal colon]*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was then incubated with ab11080, 1/1000 dilution, for 15 mins at room temperature. A goat anti-mouse biotinylated secondary antibody (ab6788, 1/1000 dilution), was used to detect the primary, and visualized using an HRP conjugated ABC system. Streptavidin HRP was used, ab7403 at a 1/10000 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• 

  Western blot - Anti-Histone H1.0 antibody [27] (ab11080)

  All lanes : Anti-Histone H1.0 antibody [27] (ab11080) at 1/500 dilution
  
  Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
  Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
  Lane 3 : Histone H1.0 Human Recombinant Protein
  
  Lysates/proteins at 30 µg per lane.
  
  Secondary
  All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 20 kDa
  Observed band size: 30 kDawhy is the actual band size different from the predicted?
  Additional bands at: 46 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
  
  
  Exposure time: 1 minute
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody [27] (ab11080)

  IHC image of Histone H1.0 staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab11080, 7.5µg/ml overnight at +4°C. An HRP-conjugated secondary (ab97240, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

  The inset negative control image is secondary-only at 1/500 dilution.

  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody [27] (ab11080)

  IHC image of Histone H1.0 staining in Human pancreas adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11080, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

   

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.