Anti-PARK7/DJ1 antibody [malphaDJ-1/E2.19]

• 产品名称

  Anti-PARK7/DJ1抗体[malphaDJ-1/E2.19]
  参阅全部 PARK7/DJ1 一抗

• 描述

  小鼠单克隆抗体[malphaDJ-1/E2.19] to PARK7/DJ1

• 宿主

  Mouse

• 经测试应用

  适用于: Flow Cyt (Intra), WB, ICCmore details

• 种属反应性

  与反应: Human
  预测可用于: Zebrafish不与反应: Mouse

• 免疫原

  Recombinant full length protein corresponding to Human PARK7/DJ1.

• 阳性对照

• WB: HeLa whole cell lysate. Flow Cyt (Intra): HepG2 cells. ICC: HeLa cells.

• 常规说明

  This monoclonal antibody to DJ-1 has been knockout validated in Western blot. The expected band for DJ-1 was observed in wild type cells and the band was not seen in knockout cells.

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.02% Sodium azide
  Constituents: 6.97% L-Arginine, PBS

• 浓度

• 批次浓度范围 100 µl 浓度为 1 - 5.1 mg/ml

• 纯度

  Purified IgM

• 克隆

  单克隆

• 克隆编号

  malphaDJ-1/E2.19

• 同种型

  IgM

The Abpromise guarantee

Abpromise™承诺保证使用ab11251于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 11315 Human

• Entrez Gene: 449674 Zebrafish

• Omim: 602533 Human

• SwissProt: Q99497 Human

• SwissProt: Q5XJ36 Zebrafish

• Unigene: 419640 Human

• Unigene: 85181 Zebrafish

• 别名

• DJ1 protein antibody

• Epididymis secretory sperm binding protein Li 67p antibody

• FLJ27376 antibody

• FLJ34360 antibody

• FLJ92274 antibody

• HEL S 67p antibody

• Oncogene DJ1 antibody

• OTTHUMP00000001348 antibody

• OTTHUMP00000001349 antibody

• OTTHUMP00000001350 antibody

• OTTHUMP00000001351 antibody

• PARK7 antibody

• PARK7_HUMAN antibody

• Parkinson disease (autosomal recessive, early onset) 7 antibody

• Parkinson disease protein 7 antibody

• Parkinson protein 7 antibody

• Protein DJ-1 antibody

• SP22 antibody

• CAP1 antibody

• DJ-1 antibody

• DJ1 antibody

• 

  Immunocytochemistry - Anti-PARK7/DJ1 antibody [malphaDJ-1/E2.19] (ab11251)

  ab11251 staining PARK7/DJ1 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab11251 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150121, Goat polyclonal Secondary Antibody to Mouse IgM - mu chain (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

  Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

• 

  Western blot - Anti-PARK7/DJ1 antibody [malphaDJ-1/E2.19] (ab11251)

  Anti-PARK7/DJ1 antibody [malphaDJ-1/E2.19] (ab11251) at 1/500 dilution + HeLa whole cell lysate at 20 µg
  

• 

  Western blot - Anti-PARK7/DJ1 antibody [malphaDJ-1/E2.19] (ab11251)

  Western blot using clone malphaDJ-1/E2.19 and a beta actin antibody as a loading control.

  The bottom band is PARK7/DJ1, the top band is beta actin.

  Lane 1: 293 cell lysate
  Lane 2: MCF-7 cell lysate
  Lanes 3-7: various different prostate cell lines
  Lane 8: recombinant PARK7/DJ1 (that was used as immunogen for this antibody)

• 

  Western blot - Anti-PARK7/DJ1 antibody [malphaDJ-1/E2.19] (ab11251)

  Lane 1: Wild-type HAP1 cell lysate (20 µg)
  Lane 2: PARK7/DJ1 knockout HAP1 cell lysate (20 µg)
  Lane 3: HeLa cell lysate (20 µg)
  Lane 4: Human brain tissue lysate (20 µg)
  Lanes 1 - 4: Merged signal (red and green). Green - ab11251  observed at 24 kDa. Red - loading control, ab181602, observed at 37 kDa.

  ab11251 was shown to specifically react with PARK7/DJ1 in wild-type HAP1 cells. No band was observed when knockout samples were used. Wild-type and PARK7/DJ1 knockout samples were subjected to SDS-PAGE. ab11251 and ab181602 (loading control to GAPDH) were diluted at 1/500 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging.

• 

  Flow Cytometry (Intracellular) - Anti-PARK7/DJ1 antibody [malphaDJ-1/E2.19] (ab11251)

  Overlay histogram showing HepG2 cells stained with ab11251 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11251, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.