Anti-gamma Tubulin抗体
参阅全部 gamma Tubulin 一抗
兔多克隆抗体to gamma Tubulin
Rabbit
适用于: ICC/IF, WBmore details
与反应: Mouse, Rat, Human, Chinese hamster
预测可用于: Drosophila melanogaster, Schizosaccharomyces pombe, Sea urchin
Synthetic peptide:
EEFATEGTDRKDVFFY-C
conjugated to KLH, corresponding to N terminal amino acids 38-53 of Human gamma Tubulin
ICC/IF: Rat2 cells; NIH3T3 cells; HeLa cells. WB: Rat2 cell lysate; A431 cell lysate; HeLa cell lysate; Jurkat cell lysate; U87 cell lysate; C2C12 cell lysate; CHO cell lysate; NIH3T3 cell lysate.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 0.42% Potassium phosphate, 0.87% Sodium chloride
浓度
批次浓度范围 50 µl 浓度为 10.5 - 12.6 mg/ml
IgG fraction
多克隆
IgG
Abpromise™承诺保证使用ab11317于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF | (5) | 1/5000. It is recommended to fix cells with methanol/acetone. |
WB | (7) | 1/1000. Detects a band of approximately 48 kDa. This concentration was determined for A431 cultured cells extract. Block with 5% BSA in TBST (not milk). Detects a band of approximately 48 kDa. Additional bands can occasionally be seen. |
Entrez Gene: 35199 Drosophila melanogaster
Entrez Gene: 27175 Human
Entrez Gene: 7283 Human
Entrez Gene: 103733 Mouse
Entrez Gene: 103768 Mouse
Omim: 191135 Human
Omim: 605785 Human
SwissProt: P42271 Drosophila melanogaster
SwissProt: P23258 Human
SwissProt: Q9NRH3 Human
SwissProt: P83887 Mouse
SwissProt: Q8VCK3 Mouse
Unigene: 469 Drosophila melanogaster
Unigene: 708059 Human
Unigene: 142348 Mouse
Gamma 1 tubulin antibody
Gamma 2 tubulin antibody
Gamma Tubulin 1 antibody
Gamma Tubulin 2 antibody
Gamma tubulin complex component 1 antibody
Gamma-2-tubulin antibody
GCP 1 antibody
GCP-1 antibody
GCP1 antibody
MGC131994 antibody
TBG2_HUMAN antibody
TUBG antibody
TUBG1 antibody
TUBG2 antibody
TUBGCP1 antibody
Tubulin gamma 1 chain antibody
Tubulin gamma 2 chain antibody
Tubulin gamma complex-associated protein 1 antibody
Tubulin gamma-2 chain antibody
tubulin, gamma 1 antibody
tubulin, gamma 2 antibody
tubulin, gamma polypeptide antibody
Xgam antibody
Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody (ab11317)
Immunocytochemistry/Immunofluorescence analysis of NIH3T3 cells labelling gamma Tubulin (green) with ab11317 at a dilution of 1/1500. A Atto448 dye conjugated goat anti-rabbit IgG was used as the seondary antibody. Actin was stained with phalloidin rhodamine conjugate (red). Nuclear staining with DAPI (blue).
Western blot - Anti-gamma Tubulin antibody (ab11317)
All lanes : Anti-gamma Tubulin antibody (ab11317) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : C2C12 cell lysate
Lane 5 : Rat2 cell lysate
Lane 6 : U87 cell lysate
Lane 7 : NIH3T3 cell lysate
Lane 8 : CHO cell lysate
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG
Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody (ab11317)
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling gamma Tubulin (green) with ab11317 at a dilution of 1/1500. A Atto448 dye conjugated goat anti-rabbit IgG was used as the seondary antibody. Actin was stained with phalloidin rhodamine conjugate (red). Nuclear staining with DAPI (blue).
Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody (ab11317)
Immunocytochemistry/Immunofluorescence analysis of Rat2 cells labelling gamma Tubulin (green) with ab11317 at a dilution of 1/1500. A Atto448 dye conjugated goat anti-rabbit IgG was used as the seondary antibody. Actin was stained with phalloidin rhodamine conjugate (red). Nuclear staining with DAPI (blue).