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Anti-gamma Tubulin antibody

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  • 产品名称

    Anti-gamma Tubulin抗体
    参阅全部 gamma Tubulin 一抗

  • 描述

    兔多克隆抗体to gamma Tubulin

  • 宿主

    Rabbit

  • 经测试应用

    适用于: ICC/IFWBmore details

  • 种属反应性

    与反应: Mouse, Rat, Human, Chinese hamster
    预测可用于: Drosophila melanogaster, Schizosaccharomyces pombe, Sea urchin

  • 免疫原

    Synthetic peptide:

    EEFATEGTDRKDVFFY-C

    conjugated to KLH, corresponding to N terminal amino acids 38-53 of Human gamma Tubulin

    Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

  • 阳性对照

    • ICC/IF: Rat2 cells; NIH3T3 cells; HeLa cells. WB: Rat2 cell lysate; A431 cell lysate; HeLa cell lysate; Jurkat cell lysate; U87 cell lysate; C2C12 cell lysate; CHO cell lysate; NIH3T3 cell lysate.

  • 常规说明


    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • 存储溶液

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituents: 0.0268% PBS, 0.42% Potassium phosphate, 0.87% Sodium chloride

  • 浓度

    • 批次浓度范围 50 µl 浓度为 10.5 - 12.6 mg/ml

  • 纯度

    IgG fraction

  • 克隆

    多克隆

  • 同种型

    IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab11317于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用Ab评论说明
ICC/IF(5)

1/5000. 

It is recommended to fix cells with methanol/acetone.

WB(7)

1/1000. Detects a band of approximately 48 kDa. This concentration was determined for A431 cultured cells extract. Block with 5% BSA in TBST (not milk). Detects a band of approximately 48 kDa. Additional bands can occasionally be seen.

数据库链接

别名

  • Gamma 1 tubulin antibody

  • Gamma 2 tubulin antibody

  • Gamma Tubulin 1 antibody

  • Gamma Tubulin 2 antibody

  • Gamma tubulin complex component 1 antibody

  • Gamma-2-tubulin antibody

  • GCP 1 antibody

  • GCP-1 antibody

  • GCP1 antibody

  • MGC131994 antibody

  • TBG2_HUMAN antibody

  • TUBG antibody

  • TUBG1 antibody

  • TUBG2 antibody

  • TUBGCP1 antibody

  • Tubulin gamma 1 chain antibody

  • Tubulin gamma 2 chain antibody

  • Tubulin gamma complex-associated protein 1 antibody

  • Tubulin gamma-2 chain antibody

  • tubulin, gamma 1 antibody

  • tubulin, gamma 2 antibody

  • tubulin, gamma polypeptide antibody

  • Xgam antibody

  • Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody (ab11317)

    Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody (ab11317)

    Immunocytochemistry/Immunofluorescence analysis of NIH3T3 cells labelling gamma Tubulin (green) with ab11317 at a dilution of 1/1500. A Atto448 dye conjugated goat anti-rabbit IgG was used as the seondary antibody. Actin was stained with phalloidin rhodamine conjugate (red). Nuclear staining with DAPI (blue).

  • Western blot - Anti-gamma Tubulin antibody (ab11317)

    Western blot - Anti-gamma Tubulin antibody (ab11317)

    All lanes : Anti-gamma Tubulin antibody (ab11317) at 1/1000 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : C2C12 cell lysate
    Lane 5 : Rat2 cell lysate
    Lane 6 : U87 cell lysate
    Lane 7 : NIH3T3 cell lysate
    Lane 8 : CHO cell lysate

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG

  • Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody (ab11317)

    Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody (ab11317)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling gamma Tubulin (green) with ab11317 at a dilution of 1/1500. A Atto448 dye conjugated goat anti-rabbit IgG was used as the seondary antibody. Actin was stained with phalloidin rhodamine conjugate (red). Nuclear staining with DAPI (blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody (ab11317)

    Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody (ab11317)

    Immunocytochemistry/Immunofluorescence analysis of Rat2 cells labelling gamma Tubulin (green) with ab11317 at a dilution of 1/1500. A Atto448 dye conjugated goat anti-rabbit IgG was used as the seondary antibody. Actin was stained with phalloidin rhodamine conjugate (red). Nuclear staining with DAPI (blue).