Anti-MAP2抗体[HM-2]
参阅全部 MAP2 一抗
小鼠单克隆抗体[HM-2] to MAP2
Mouse
适用于: ICC, WBmore details
与反应: Rat
预测可用于: Mouse, Chicken, Cow, Human, Quail
Full length protein corresponding to Rat MAP2. Rat brain microtubule associated proteins (MAPs).
WB: Rat brain tissue lysate. ICC: B35 cells.
This product was changed from ascites to tissue culture supernatant on 05/06/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS
Tissue culture supernatant
单克隆
HM-2
IgG1
Abpromise™承诺保证使用ab11267于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC | (2) | Use a concentration of 2 µg/ml. Fix cells in 4% paraformaldehyde/PBS for 45 min; then permeabilise cells with 0.2% Triton X-100 in PBS for 5 min (see Farah et al) OR fix in 4% paraformaldehyde (containing 0.2% picric acid in 0.1 M phosphate buffer, pH 6.9) for 15 min at room temperature (see O' Hare et al) . Read More |
| WB | (2) | Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 200 kDa. |
Entrez Gene: 4133 Human
Entrez Gene: 17756 Mouse
Omim: 157130 Human
SwissProt: P11137 Human
SwissProt: P20357 Mouse
Unigene: 368281 Human
Unigene: 256966 Mouse
Unigene: 436793 Mouse
Unigene: 10484 Rat
Unigene: 136301 Rat
Unigene: 230443 Rat
DKFZp686I2148 antibody
MAP 2 antibody
MAP dendrite specific antibody
MAP-2 antibody
MAP2 antibody
MAP2A antibody
MAP2B antibody
MAP2C antibody
Microtubule associated protein 2 antibody
Microtubule-associated protein 2 antibody
MTAP2_HUMAN antibody
![Immunocytochemistry - Anti-MAP2 antibody [HM-2] (ab11267)](https://www.abcam.cn/ps/products/11/ab11267/Images/ab11267-320126-anti-map2-antibody-hm-2-immunofluorescence.jpg)
Immunocytochemistry - Anti-MAP2 antibody [HM-2] (ab11267)Bailey, J.A. et al PLoS One. 2011;6(7):e21954. doi: 10.1371/journal.pone.0021954. Epub 2011 Jul 22 Reproduced under the Creative Commons license https://creativecommons.org/publicdomain/zero/1.0/
Rivastigmine preserves neuronal morphology and alters sAPP secretion
At day 12 or day 20, cells were fixed for immunocytochemistry analysis and probed with anti-MAP2 (green) and anti-GFAP (red) antibodies. Neuronal MAP2 immunoreactivity declines to almost undetectable levels between day 12 and day 20 in untreated cells, whereas neuronal morphology is preserved in rivastigmine treated cultures. Glial GFAP was observed to increase in both treated and untreated cells between day 12 and day 20. These results confirm the degeneration of neurons by day 20.
MAP2 is detected using ab11267 in 4% paraformaldehyde-fixed, 0.5% Triton X-100 permeabilized embryonic rat cerebrocortical cells.
(From Figure 2A of Bailey et al)
This image was generated using the ascites version of the product.
![Western blot - Anti-MAP2 antibody [HM-2] (ab11267)](https://www.abcam.cn/ps/products/11/ab11267/Images/ab11267-320127-267b.jpg)
Western blot - Anti-MAP2 antibody [HM-2] (ab11267)
Anti-MAP2 antibody [HM-2] (ab11267) at 1 µg/ml + Rat brain lysate
Predicted band size: 200 kDa
This image was generated using the ascites version of the product.
![Immunocytochemistry - Anti-MAP2 antibody [HM-2] (ab11267)](https://www.abcam.cn/ps/products/11/ab11267/Images/ab11267-251913-anti-map2-antibody-hm-2-immunofluorescence.jpg)
Immunocytochemistry - Anti-MAP2 antibody [HM-2] (ab11267)
Immunocytochemical analysis of B35 (rat neuroblastoma cell line) cells labelling MAP2 with ab11267 at a concentration of 2 μg/mL. The secondary was developed using Goat anti-mouse IgG. Cells were counterstained with DAPI to stain nuclei.
This image was generated using the ascites version of the product.
