Anti-Connexin 43 / GJA1抗体- Intercellular Junction Marker
参阅全部 Connexin 43 / GJA1 一抗
兔多克隆抗体to Connexin 43 / GJA1 - Intercellular Junction Marker
Rabbit
适用于: IHC-Fr, ICC, IHC-P, WBmore details
与反应: Mouse, Rat, Hamster, Cow, Dog, Human, Pig, Monkey
Synthetic peptide corresponding to Human Connexin 43/ GJA1 aa 362-382 (C terminal) conjugated to keyhole limpet haemocyanin.
Sequence:
KPSSRASSRASSRPRPDDLEI
Database link: P17302
WB: Mouse brain and heart tissue extract; HEK-293, P19 and Rat2 cell lysates. IHC-P: Cow heart tissue; human heart and testis tissue; mouse heart muscle tissue. ICC: BHK cells. Human pluripotent stem cell derived cardiomyocyte.
Storage in frost-free freezers is not recommended.
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077). Or search our wide range of secondary antibodies for use with your experiment.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 1% BSA
浓度
批次浓度范围 50 µl 浓度为 0.6 - 0.7 mg/ml
Immunogen affinity purified
Affinity isolated antigen specific antibody is obtained by immunospecific purification which removes essentially all rabbit serum proteins, including immunoglobulins, which do not specifically bind to connexin 43.
多克隆
IgG
Abpromise™承诺保证使用ab11370于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr | (2) | Use at an assay dependent concentration. |
| ICC | (1) | 1/400. |
| IHC-P | (19) | 1/1000 - 1/5000. |
| WB | (6) | 1/2000 - 1/8000. Detects a band of approximately 43 kDa. |
Entrez Gene: 2697 Human
Entrez Gene: 14609 Mouse
Omim: 121014 Human
SwissProt: P17302 Human
SwissProt: P23242 Mouse
Unigene: 74471 Human
Unigene: 378921 Mouse
Unigene: 10346 Rat
Connexin 43 antibody
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Cx 43 antibody
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Gap junction 43 kDa heart protein antibody
Gap junction alpha-1 protein antibody
Gap junction protein alpha 1 43kDa (connexin 43) antibody
Gap junction protein alpha 1 43kDa antibody
Gap junction protein alpha like antibody
GJA 1 antibody
Gja1 antibody
GJAL antibody
ODD antibody
ODDD antibody
ODOD antibody
SDTY3 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)This image is courtesy of an anonymous Abreview
Immunohistochemistry analysis (Formalin/PFA-fixed paraffin-embedded sections) of paraformaldehyde fixed mouse spleen tissue. Stained with ab11370 at 1/1000 dilution. Secondary antibody used was Dako EnVision+ System- HRP Labelled Polymer Anti-R. Blocking was done with 5% serum for 1 hour at 21°C. The sample was incubated with the primary antibody and 5% Normal Goat Serum for 19 hours at 4°C. Antigen retrieval method was heat mediated Tris/EDTA pH 9.0
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)This image is courtesy of an anonymous Abreview
Immunohistochemistry analysis (Formalin/PFA-fixed paraffin-embedded sections) of paraformaldehyde fixed human palatine tonsil. Stained with ab11370 at 1/1000 dilution. Secondary antibody used was Dako EnVision+ System- HRP Labelled Polymer Anti-R. Blocking was done with 5% serum for 1 hour at 21°C. The sample was incubated with the primary antibody and 5% Normal Goat Serum for 19 hours at 4°C. Antigen retrieval method was heat mediated Tris/EDTA pH 9.0
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)This image is courtesy of an anonymous Abreview
Immunohistochemistry analysis (Formalin/PFA-fixed paraffin-embedded sections) of paraformaldehyde fixed rat heart (cardiac muscle tissue). Stained with ab11370 at 1/1000 dilution. Secondary antibody used was Dako EnVision+ System- HRP Labelled Polymer Anti-R. Blocking was done with 5% serum for 1 hour at 21°C. The sample was incubated with the primary antibody and 5% Normal Goat Serum for 19 hours at 4°C. Antigen retrieval method was heat mediated Tris/EDTA pH 9.0
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)
Immunohistochemical analysis of formalin-fixed, paraffion-embedded cow heart tissue staining Connexin 43 / GJA1 using ab11370 at1/2000 dilution. Detected using an Anti-Rabbit IgG-biotin antibody and an Avidin-peroxidase conjugate.
Western blot - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)
All lanes : Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370) at 1/2000 dilution
Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : P19 (mouse embryonic carcinima cell line) cell lysate
Lane 3 : Rat2 (rat embryo-derived cell line) cell lysate
Lane 4 : MDCK (canine kidney cell line) cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG-peroxidase conjugate
Western blot - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)
Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370) at 1/8000 dilution + Mouse brain extract
Secondary
Anti-Rabbit IgG-AP conjugate with NBT/BCIP substrate
Immunocytochemistry - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)
Immunocytochemical analysis of BHK (baby hamster kidney cell line) cells fixed and permeabilized with methanol followed by methanol:acetone. -Connexin 43 / GJA1 was labeled using ab11370 at 1/400 dilution, followed by a Goat Anti-Rabbit-FITC conjugate (Green). The nuclear counterstain is DAPI (Blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)
Immunohistochemical analysis of formalin-fixed, paraffion-embedded human heart tissue staining Connexin 43 / GJA1 using ab11370 at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG-FITC conjugate (Green).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)This image is courtesy of Carl Hobbs, King's College London, United Kingdom
Immunohistochemical detection of Connexin 43 / GJA1 using ab11370 (1/1000) on human testis sections (PFA-fixed paraffin-embedded sections). Antigen retrieval : Heat mediated - Buffer/Enzyme Used: Citric acid pH6, 1% BSA as blocking agent for 10 mins @ rt°C. Biotin labelled secondary antibody used at 1/200. Stained are clusters of Leydig cells in the interstitium and what should be Sertoli cells within the seminiferous tubules and the junction between (?). Sertoli cells and tubule "capsular" cells are intensely positive at their membrane interfaces (capsular interface positivity only evident in upper middle of image). Interestingly,smooth muscle of human colon and myometrium were completely negative when tested at the same time.
Western blot - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)Image courtesy of an anonymous Abreview.
All lanes : Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370) at 1/6000 dilution
Lane 1 : Whole tissue lysate prepared from murine left ventricle at 5 µg
Lane 2 : Whole tissue lysate prepared from murine left ventricle at 10 µg
Lane 3 : Whole tissue lysate prepared from murine left ventricle at 20 µg
Lane 4 : Whole tissue lysate prepared from murine left ventricle at 30 µg
Lane 5 : Whole tissue lysate prepared from murine left ventricle at 40 µg
Secondary
All lanes : HRP-conjugated donkey anti-rabbit polyclonal at 1/10000 dilution
Developed using the ECL technique.
Observed band size: 43 kDawhy is the actual band size different from the predicted?
Exposure time: 30 seconds
Immunocytochemistry - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)This image is courtesy of an anonymous Abreview
ab11370 staining Connexin 43 / GJA1 in human pluripotent stem cell derived cardiomyocyte by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with saponin and blocked with 5% serum for 15 minutes at room temperature. Samples were incubated with primary antibody (1/1000) for 16 hours at 4°C. An Alexa Fluor® 568-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
Clear antibody signal (yellow) at the interface of adjoining cells in a population of human embryonic stem cell derived cardiomyocytes (red = sarcomeric alpha actinin).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)This image is courtesy of Carl Hobbs, King's College London, United Kingdom
Immunohistochemistical detection of Connexin 43 / GJA1 using ab11370 on Formaldehyde-fixed paraffin-embedded mouse heart muscle sections. Antigen retrieval step: heat mediated using citric acid pH6. Blocking step: 1% BSA for 10 mins @ rt°C. Primary antibody ab11370 incubated at 1/2000 for 2 hours. Secondary antibody: anti rabbit IgG conjugated to biotin used at 1/200. Submitted image of cardiac myofibres in L/S shows clear, specific labelling of Intercalated discs which are rich in Gap junctions (Connexin 43 is a major component of Gap junctions). No other positivity is observed in mouse heart (the image is taken from a whole heart section). NB: in mouse tongue the ventral keratinising stratified squamous epithelium shows positivity in not only basal cells but also in the Prickle cell layer, although markedly reduced: data not shown but see here http://www.immunoportal.com/ for appropriate image.
