Anti-Integrin alpha V antibody [P2W7]

• 产品名称

  Anti-Integrin alpha V抗体[P2W7]
  参阅全部 Integrin alpha V 一抗

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha V antibody [P2W7] (ab11470)

  ICC/IF image of ab11470 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11470, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, a goat anti-mouse DyLight® 488 (IgG; H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• 

  Flow Cytometry - Anti-Integrin alpha V antibody [P2W7] (ab11470)

  Overlay histogram showing A549 cells stained with ab11470 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11470, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed A549 cells used under the same conditions.
  
  Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

• 描述

  小鼠单克隆抗体[P2W7] to Integrin alpha V

• 宿主

  Mouse

• 经测试应用

  适用于: ICC/IF, Flow Cytmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Tissue, cells or virus corresponding to Human Integrin alpha V. Ocular melanoma cell line (V+B2) a clone expressing high levels of alpha-v, beta-1
  Database link: P06756

• 阳性对照

• Tested with squamous cell carcinoma cells. A375M melanoma cell line. A549 cell line IF/ICC

• 常规说明

  Human alpha v is about 150-160kDa under non-reducing conditions. Under reducing conditions it is cleaved into 2 bands of about 125-130kDa +20-25kDa.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.02% Sodium azide
  Constituent: 99.98% PBS

• 浓度

• 50 µg 浓度为 1 mg/ml

• 纯度

  Protein A purified

• 纯化说明

  The antibody is Protein A purified from tissue culture supernatant.

• 克隆

  单克隆

• 克隆编号

  P2W7

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab11470于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 3685 Human

• Omim: 193210 Human

• SwissProt: P06756 Human

• Unigene: 436873 Human

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