Anti-ErbB2 / HER2 antibody [ICR12]

• 产品名称

  Anti-ErbB2 / HER2抗体[ICR12]
  参阅全部 ErbB2 / HER2 一抗

• 描述

  大鼠单克隆抗体[ICR12] to ErbB2 / HER2

• 宿主

  Rat

• 经测试应用

  适用于: IHC-P, ICC/IF, Flow Cytmore details
  不适用于: IP or WB

• 种属反应性

  与反应: Human
  

• 免疫原

  Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

• 阳性对照

• IHC-P: Human breast carcinoma tissue. ICC/IF: SK-BR-3 cells. Flow Cyt: SK-BR-3 cells.

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  This product is a recombinant monoclonal antibody, which offers several advantages including:

  For more information see here.

• - High batch-to-batch consistency and reproducibility

• - Improved sensitivity and specificity

• - Long-term security of supply

• - Animal-free production

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.2
  Preservative: 0.01% Sodium azide
  Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)

• 浓度

• 100 µg 浓度为 1.038 mg/ml

• 纯度

  Ion Exchange Chromatography

• 克隆

  单克隆

• 克隆编号

  ICR12

• 同种型

  IgG2a

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab11710于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用说明

Is unsuitable for IP or WB.

• 数据库链接

• Entrez Gene: 2064 Human

• Omim: 164870 Human

• SwissProt: P04626 Human

• Unigene: 446352 Human

• 别名

• CD340 antigen antibody

• Cerb B2/neu protein antibody

• CerbB2 antibody

• Erb b2 receptor tyrosine kinase 2 antibody

• ErbB-2 proto-oncogene antibody

• ERBB2 antibody

• ERBB2_HUMAN antibody

• HER 2 antibody

• HER 2/NEU antibody

• HER2 antibody

• Herstatin antibody

• Human epidermal growth factor receptor 2 antibody

• Metastatic lymph node gene 19 protein antibody

• MLN 19 antibody

• MLN19 antibody

• NEU antibody

• NEU proto oncogene antibody

• Neuro/glioblastoma derived oncogene homolog antibody

• Neuroblastoma/glioblastoma derived oncogene homolog antibody

• NGL antibody

• p185erbB2 antibody

• Proto-oncogene c-ErbB-2 antibody

• Proto-oncogene Neu antibody

• Receptor tyrosine-protein kinase erbB-2 antibody

• TKR1 antibody

• Tyrosine kinase type cell surface receptor HER2 antibody

• Tyrosine kinase-type cell surface receptor HER2 antibody

• V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog) antibody

• V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 antibody

• V erb b2 avian erythroblastic leukemia viral oncoprotein 2 antibody

• V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) antibody

• V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody

• Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) antibody

• Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody

• C erb B2/neu protein antibody

• CD340 antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [ICR12] (ab11710)

  Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling ErbB2 / HER2 with ab11710 at 1/400 dilution followed by ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Membranous staining on human breast carcinoma. Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

  Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• 

  Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 antibody [ICR12] (ab11710)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SK-BR-3 cells labelling ErbB 2 with ab11710 at 1/100 dilution, followed by ab150157 Goat Anti-rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing strong membranous staining in SK-BR-3 cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/1000 diliution (Red). The nuclear counterstain was DAPI (Blue).

  Negative control 1: ab11710 at 1/100 dilution followed by ab150080 at a 1/1000 dilution.

  Negative control 2: ab179513 at a 1/200 dilution followed by ab150157 at a 1/1000 dilution.

  Negative control cells: MCF7 (PMID: 18288420). 

• 

  Flow Cytometry - Anti-ErbB2 / HER2 antibody [ICR12] (ab11710)

  Flow cytometric analysis of MCF7 (human breast adenocarcinoma epithelial cell, Left panel) / SK-BR-3 (human breast adenocarcinoma epithelial cell, Right panel) cells labelling ErbB 2 with ab11710 at 1/1000 dilution (0.1µg) (Red) compared with a rat monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rat IgG (Alexa Fluor® 488, ab150157) at 1/2000 dilution was used as the secondary antibody.

  Low expression control: MCF7 (PMID: 17938260).

  Gated on viable cells.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [ICR12] (ab11710)

  Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling ErbB 2 with ab11710 at 1/400 dilution followed by ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). No staining on human breast carcinoma without expression of HER2 is observed. Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

  Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• 

  Anti-ErbB2 / HER2 antibody [ICR12] (ab11710)