Anti-gamma Tubulin抗体
参阅全部 gamma Tubulin 一抗
兔多克隆抗体to gamma Tubulin
Rabbit
适用于: ICC, WBmore details
与反应: Mouse, Rat, Dog, Human, Chinese hamster
Synthetic peptide:
AATRPDYISWGTQEQ-CG
, with N-terminally added cysteine-glycine, conjugated to KLH, corresponding to amino acids 437-451 of Xenopus laevis gamma Tubulin.
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS
IgG fraction
多克隆
IgG
Abpromise™承诺保证使用ab11321于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC | 1/1000. | |
| WB | (1) | 1/1000. Predicted molecular weight: 51 kDa. |
Entrez Gene: 27175 Human
Entrez Gene: 7283 Human
Entrez Gene: 103733 Mouse
Entrez Gene: 103768 Mouse
Omim: 191135 Human
Omim: 605785 Human
SwissProt: P23258 Human
SwissProt: Q9NRH3 Human
SwissProt: P83887 Mouse
SwissProt: Q8VCK3 Mouse
Unigene: 708059 Human
Unigene: 142348 Mouse
Gamma 1 tubulin antibody
Gamma 2 tubulin antibody
Gamma Tubulin 1 antibody
Gamma Tubulin 2 antibody
Gamma tubulin complex component 1 antibody
Gamma-2-tubulin antibody
GCP 1 antibody
GCP-1 antibody
GCP1 antibody
MGC131994 antibody
TBG2_HUMAN antibody
TUBG antibody
TUBG1 antibody
TUBG2 antibody
TUBGCP1 antibody
Tubulin gamma 1 chain antibody
Tubulin gamma 2 chain antibody
Tubulin gamma complex-associated protein 1 antibody
Tubulin gamma-2 chain antibody
tubulin, gamma 1 antibody
tubulin, gamma 2 antibody
tubulin, gamma polypeptide antibody
Xgam antibody
Immunocytochemistry - Anti-gamma Tubulin antibody (ab11321)
Immunocytochemistry analysis of Rat 1 cells labeling gamma Tubulin with ab11321 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, Cy3™ conjugate. Cells were fixed and permeabilized with methanol followed by acetone.
Western blot - Anti-gamma Tubulin antibody (ab11321)
All lanes : Anti-gamma Tubulin antibody (ab11321) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : NIH-3T3 cell lysate
Lane 3 : MDCK cell lysate
Lane 4 : CHO cell lysate
Lane 5 : Rat-2 cell lysate
Lane 6 : A431 cell lysate
Lane 7 : Jurkat cell lysate
Lane 8 : U87 cell lysate
Lane 9 : C2C12 cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG-peroxidase
Predicted band size: 51 kDa
Immunocytochemistry - Anti-gamma Tubulin antibody (ab11321)
Immunocytochemistry analysis of HeLa cells labeling gamma Tubulin with ab11321 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, Atto-488 conjugate. Cells were fixed and permeabilized with methanol followed by acetone.
Immunocytochemistry - Anti-gamma Tubulin antibody (ab11321)
ICC/IF image of ab11321 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11321, 5 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek293, HepG2 and MCF7 cells.
Western blot - Anti-gamma Tubulin antibody (ab11321)
All lanes : Anti-gamma Tubulin antibody (ab11321) at 1/1000 dilution
Lane 1 : Mouse liver tissue lysate
Lane 2 : Mouse hepatocytes whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-rabbit (HRP) secondary antibody at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 50 kDawhy is the actual band size different from the predicted?
Exposure time: 5 seconds
Blocking buffer 5% milk.
