Anti-Pit1 antibody [5E4]

• 产品名称

  Anti-Pit1抗体[5E4]
  参阅全部 Pit1 一抗

• 描述

  小鼠单克隆抗体[5E4] to Pit1

• 宿主

  Mouse

• 经测试应用

  适用于: WB, ICC/IF, IPmore details

• 种属反应性

  与反应: Rat
  预测可用于: Sheep, Cow, Human, Pig, Macaque monkey

• 免疫原

  Recombinant fragment:

  SAAECLPASN HATNVMSTAT GLHYSVPSCH YGNQPSTYGV MAGSLTPCLY KFPDHTLSHG FPPLHQPLLA EDPAASEFKQ ELRRKSKLVEE PIDMDSPEIR ELEQFANEFK VRRIKL

  , corresponding to amino acids 30-146 of Rat Pit1.

  Run BLAST with Run BLAST with 

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• 纯度

  Tissue culture supernatant

• 克隆

  单克隆

• 克隆编号

  5E4

• 骨髓瘤

  NS1

• 同种型

  IgG2a

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab10545于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 282315 Cow

• Entrez Gene: 5449 Human

• Entrez Gene: 25517 Rat

• Entrez Gene: 397325 Sheep

• Omim: 173110 Human

• SwissProt: P28069 Human

• SwissProt: Q04788 Pig

• SwissProt: P10037 Rat

• SwissProt: P79364 Sheep

• Unigene: 591654 Human

• Unigene: 10445 Rat

别名

• Dwarf antibody

• GHF 1 antibody

• GHF 1A antibody

• GHF-1 antibody

• GHF1 antibody

• GHF1A antibody

• Growth hormone factor 1 antibody

• Hmp 1 antibody

• Hmp1 antibody

• Pit 1 antibody

• Pit 1 beta antibody

• PIT 1Z antibody

• PiT-1 antibody

• Pit1 beta antibody

• PIT1_HUMAN antibody

• PIT1Z antibody

• Pituitary growth hormone antibody

• Pituitary specific positive transcription factor 1 antibody

• Pituitary-specific positive transcription factor 1 antibody

• POU class 1 homeobox 1 antibody

• POU domain class 1 transcription factor 1 antibody

• POU domain transcriptional regulator antibody

• POU1F1 antibody

• 

  Western blot - Anti-Pit1 antibody [5E4] (ab10545)

  At 1:40 dilution the western was very good on GH3 nuclear lysate which has an abundant expression of Pit1. At 1:40 dilution the western was very good on GH3 nuclear lysate which has an abundant expression of Pit1.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Pit1 antibody [5E4] (ab10545)This image is courtesy of Zelton Dave Sharp

  IF staining on rat samples using ab10545 carried out as per the reference (see below) by Zelton Dave Sharp of The University of Texas, San Antonio.

• 

  Immunoprecipitation - Anti-Pit1 antibody [5E4] (ab10545)

  Antibody to Pit1 (ab10545) detects a 33 kDa band in IP followed by WB.

  Two IgG monoclonal antibodies were used to immunoprecipitate pituitary-derived GH3 cellular proteins, and followed by Western analysis. Whole cell extracts (WCE) and immunoprecipitated fractions contain a prominent 33 kD band representing Pit-1. Nonspecific hybridoma supernatant was used as a negative control. Epitope mapping (data not shown) indicated that both IgG monoclonal antibodies recognize an epitope in the distal N-terminus within the ST activation domain of Pit-1