Anti-CREBBP抗体
参阅全部 CREBBP 一抗
兔多克隆抗体to CREBBP
Rabbit
适用于: IP, WBmore details
与反应: Human
预测可用于: Chimpanzee
Synthetic peptide corresponding to Human CREBBP. Within exon 17.
Lysate from HeLa cells.
Cyclic AMP-responsive enhancer binding protein (CREB) binding protein (CBP) and p300 are closely related transcriptional coactivators that have been shown to directly interact with many different DNA-binding transcription factors including nuclear hormone receptors, CREB (cyclic AMP-responsive enhancer binding protein), c-Fos, c-Jun/v-Jun, c-Myb/v-Myb, TFIIB and MyoD.Both CBP and p300 have been shown to display histone acetyltransferase (HAT) activity, capable of acetylating all four core histone particles in nucleosomes.As a result of HAT activity, it has been suggested CBP and p300 may play a direct role in activating chromatin for transcription.Single point mutations in CBP have been proposed as causative factors in the developmental abnormalities of Rubinstein-Taybi syndrome (RTS).Although both CBP and p300 appear to function similarly, the inability of p300 to rescue CBP malfunction iRTS suggests intrinsic functional differences between CBP and p300.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Immunogen affinity purified
Antibodies were affinity purified using the peptide immobilized on solid support.
Cyclic AMP-responsive enhancer binding protein (CREB) binding protein (CBP) and p300 are closely related transcriptional coactivators that have been shown to directly interact with many different DNA-binding transcription factors including nuclear hormone receptors, CREB (cyclic AMP-responsive enhancer binding protein), c-Fos, c-Jun/v-Jun, c-Myb/v-Myb, TFIIB and MyoD.Both CBP and p300 have been shown to display histone acetyltransferase (HAT) activity, capable of acetylating all four core histone particles in nucleosomes.As a result of HAT activity, it has been suggested CBP and p300 may play a direct role in activating chromatin for transcription.Single point mutations in CBP have been proposed as causative factors in the developmental abnormalities of Rubinstein-Taybi syndrome (RTS).Although both CBP and p300 appear to function similarly, the inability of p300 to rescue CBP malfunction iRTS suggests intrinsic functional differences between CBP and p300.
多克隆
IgG
Abpromise™承诺保证使用ab10489于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | Use a concentration of 1 - 4 µg/ml. | |
| WB | Use a concentration of 0.4 µg/ml. Predicted molecular weight: 265 kDa. |
Entrez Gene: 1387 Human
Omim: 600140 Human
SwissProt: Q92793 Human
Unigene: 459759 Human
CREB-binding protein antibody
Crebbp antibody
Cyclic AMP responsive enhancer binding protein antibody
KAT3A antibody
RSTS antibody
RTS antibody
Rubinstein Taybi syndrome antibody
CBP antibody
CBP_HUMAN antibody
CREB binding protein antibody
Western blot - Anti-CREBBP antibody (ab10489)
All lanes : Anti-CREBBP antibody (ab10489) at 0.4 µg/ml
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 5 µg
Predicted band size: 265 kDa
Exposure time: 3 minutes
Immunoprecipitation - Anti-CREBBP antibody (ab10489)
ab10489 immunoprecipitating CREBBP at 6 µg/ml lysate.
Lane 1: Anti-CREBBP antibody ab10489 in HeLa whole cell extract (1 mg per IP reaction; 20% of IP loaded).
Lane 2: Anti-CREBBP antibody ab10490 in HeLa whole cell extract (1 mg per IP reaction; 20% of IP loaded).
Lane 3: IgG control.
For blotting immunoprecipitated CREBBP, ab10489 was used at 1 µg/mL.
Detection: Chemiluminescence with an exposure time of 30 seconds.
