Anti-CSNK2A2抗体
参阅全部 CSNK2A2 一抗
兔多克隆抗体to CSNK2A2
Rabbit
Reacts with the alpha prime subunit.
适用于: WB, IPmore details
与反应: Mouse, Human
预测可用于: Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant
Synthetic peptide corresponding to Human CSNK2A2 aa 300 to the C-terminus.
Database link: 1459
WB: HeLa, HEK293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.
CKII is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The gene for this protein is found on chromosome 20, but a related transcribed pseudogene is found on chromosome 11. Three transcript variants have been found, encoding two different isoforms. Protein kinase CKII is recognized as one of the key cellular signals for cell growth and proliferation; it also has an important role in apoptosis.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
浓度
50 µg 浓度为 1 mg/ml
Immunogen affinity purified
Antibodies were affinity purified using the peptide immobilized on solid support.
CKII is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The gene for this protein is found on chromosome 20, but a related transcribed pseudogene is found on chromosome 11. Three transcript variants have been found, encoding two different isoforms. Protein kinase CKII is recognized as one of the key cellular signals for cell growth and proliferation; it also has an important role in apoptosis.
多克隆
IgG
Abpromise™承诺保证使用ab10474于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (4) | 1/1000 - 1/5000. Detects a band of approximately 39 kDa (predicted molecular weight: 41 kDa). |
| IP | (1) | Use at 2-10 µg/mg of lysate. |
Entrez Gene: 1459 Human
Entrez Gene: 13000 Mouse
Omim: 115442 Human
SwissProt: P19784 Human
SwissProt: O54833 Mouse
Unigene: 82201 Human
Unigene: 440348 Mouse
Unigene: 51136 Mouse
Casein kinase 2 alpha prime polypeptide antibody
Casein kinase II alpha' chain antibody
Casein kinase II subunit alpha' antibody
CK II alpha' antibody
CK II antibody
CK2A2 antibody
CKII antibody
CSK22_HUMAN antibody
CSNK2A1 antibody
CSNK2A2 antibody
FLJ43934 antibody
Western blot - Anti-CSNK2A2 antibody (ab10474)
Detection of human and mouse CSNK2A1 by western blot.
Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer.
Antibody: Affinity purified rabbit anti-CSNK2A1 antibody A300-199A (lot A300-199A-5) used for WB at 0.1 µg/ml.
Detection: Chemiluminescence
Immunoprecipitation - Anti-CSNK2A2 antibody (ab10474)
CSNK2A2 was immunoprecipitated from 1mg of HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate with ab10474 at 6 µg/ml. Western blot was performed from the immunoprecipitate using ab10474 at 1 µg/ml.
Lane 1: ab10474 IP in HeLa whole cell lysate.
Lane 2: Ctrl IgG instead of ab10474.
Western blot - Anti-CSNK2A2 antibody (ab10474)
Detection of human CSNK2A2 by Western blot and immunoprecipitation.
A. RIPA extract (30 mg) from HeLa cells treated with CSNK2A2 siRNA or vimentin siRNA (v).
B. RIPA extract (30 mg) from HeLa cells treated with CSNK2A2, CSNK2A2 prime, or vimentin (v) siRNA.
C. RIPA extract (0.5mg) from HeLa cells.
Antibody: ab10474 used at 0.33 (A) or 0.2 (B) mg/ml for WB or 5 mg/0.5 mg lysate (C) for IP.
Detection of human CSNK2A2 by Western blot and immunoprecipitation. A. RIPA extract (30 mg) from HeLa cells treated with CSNK2A2 siRNA or vimentin siRNA (v). B. RIPA extract (30 mg) from HeLa cells treated with CSNK2A2, CSNK2A2, or vimentin (v) siRNA. C. RIPA extract (0.5mg) from HeLa cells. Antibody: ab10474 used at 0.33 (A) or 0.2 (B) mg/ml for WB or 5 ug/0.5 mg lysate (C) for IP.
Western blot - Anti-CSNK2A2 antibody (ab10474)Image courtesy of an anonymous Abreview.
All lanes : Anti-CSNK2A2 antibody (ab10474) at 1/500 dilution
Lane 1 : Whole cell lysate prepared from alpha prime knock out MEFs
Lane 2 : Whole cell lysate prepared from alpha prime heterozygous MEFs
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat anti rabbit IgG-HRP at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 41 kDa
Observed band size: 37 kDawhy is the actual band size different from the predicted?
Exposure time: 30 minutes
